XB-CLONE-4352843
Clone Name: IMAGE:7027558
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Gene:
ucp2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783992 [+]
taattaatccccccccccccctcaagttccctcagtccgacttgaggacaataagttcccggcgtcacaagatctgacacaccggcccctctacccccca
ttcccgctgtggagcattccctttccatccgtaccgcataaggattatggttttaaggcatcaagtttcccgccgcgtttctatggcaagtcaagagtat
caaatatttagagccagtggcaaatcagacgggcgggacgccttcagggaatcctgatcctccagcagctctcgcagcccccctgcaatactcacctaag
acgttggcagagtgactttgccttgtcttctcactacacggcacttattctcaggaatccgctcctctccgcagagaaccgctcggcaccatggttggat
tcaagcccaccgacatcccacccacagcggcccgtcaaattcgtcggcgctgggacagctacatgtatcgccgatctgttcaccttccctctgggaacgg
ttaaagttccgactccacattcaaggagagaacaaggcggttcaacgtgaatgccggcccaaaaacaaggggaatgttttgggcaccatcttttaccctt
ggtggaaaaacagtaagggcctcaaggagttctgataccaacgggttttgattagcccgggcctgcccaccgttctaaatataacctccctctcaccccg
tacccgggatttgggaacttcgtccgaatcttcgggatcccgaggcgaaatttttttttcccccaacaatgggtatctccaccctctgtttttcacaaga
gagtgtgtggcgccgtcacacaccctccgcgaatgggaaaattctcntggtgcaccgcatgtgtgactcccacacacacctcggtgttttttatttctag
cgagaggcttttttcaacacagaacggagcggtngtcacacattattatatcggaaagtaccgccgagatatagt
3' EST: CF783991 [+]ttggtgtaaaattgcttgaccgagtcgtagagtccgatccggacggaggcgaagctcatctggcgctgcaggccggcaaccaagccgttgtacagactct
tgggcccttccgtcttcaccatggtagagatggtgccaaacactcccttgtactgggccgccttcacgttaaccaccttgttctctccttgaatctggag
tcggactttggccgtgtccagagggaaggtgaacagatcggcgatacatgctgctgtcccagcgccgacgaatttgacggccgctgtgggtgggatgtcg
gtgggcttgaatccaaccatggtgccgagcggttctctgcggagaggagcggattcctgagaataagtgccgtgtagtgagaagacaaggcaaagtcact
ctgccaacgtcttaggtgagtattgcaggggggctgcgagagctgctggaggatcaggattccctgaaggcgtcccgcccgtctgatttgccactggctc
taaatatttgatactcttgacttgccatagaaacgcggcgggaaacttgatgccttaaaaccataatccttatgcggtacggatggaaagggaatgctcc
acagcgggaatggggggtagaggggccggtgtgtcagatcttgtgacgccgggaacttattgtcctcaagtcggactgagggaacttgaggggggggggg
ggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: