XB-CLONE-4352840
Clone Name: IMAGE:7027556
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Gene:
sri
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783994 [+]
taattaatccccccccccccccgactcactctctgcagcggccatggcgtatccgggacagcagccaggaggctattaccaaggagggtatggcggagcc
cctgggggtcctgcatatggtcagcagcaagaccccatgtatggctactttgcatctattgccggccaggatggccagattgatgctgatgagctacagc
ggtgcctcacacaagcagggctctctgggggatacaaaccttttagtctggaaacttgccgacttatgatcgctatgctggatagggacatgtctggcaa
aaagggctttaccgaatttcaacaaccggggacggtaataaatggttgcacacagtattctcaagacccatgatggcgccaggagcggtccattttaagg
gcacgaacttcctgccctctttatgatcgcttgcgggtaccccaatgagccccctagcccctttaacccttcccctctaccgagaatttaccatgaaatg
gggtaagaaacccgtctttccggatgccccgccgcctcatccttgctcttactatcgacaaattggctaaaacctcttggacccatataaactggccttt
atggaccgcgcaatgggccactttccttttcccaccctattttgttcttgtcccgcacctgtgaacagcatttgatctcaccctgtatcgttcgaatgcc
catctcgtttgtctgtctatgagcaagaatttgcgctccgggcggggtatgatatgtatagttcatgatacatgtgtgcagcgagggcggtgtcgccccg
tattattccctctcgtataccacctccttttttctccgccccccccagcttctattactccctccccctgttataatccccgacgccacaccttatctat
atctccactcgcgtttgctattgtgcccacatcgttttagtgtcccgcctcacaccctatctgatttctcaccgcttttgtggaccacacacacgcacac
gtcgcttaagatatattccgggagagtggggatacaaaacgatattatatatactcccctcccagcccccttgtctctcagatatccccgcaccccccac
atatattatttgaat
3' EST: CF783993 [+]tttttttttttttttccttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
tttttttttttttttttttttttcccccccccgggggttttttttaaaaaaaaaaatttttgggaaaaaaaaaacccgggggaaaaaaaagcccccaagt
ttttttttttgggggaaaaaacggggaaaaaaagggggaccccggggttttccccccccccccccctttttttgggggggggccccctttaaaaaaaacc
cccccggggggggtccccccccccggggggggaaaccccccccccccaaaaaagggggggaaaaaccccctttttcccccgggggggggggggcccccaa
aaaaaaacccccccctgggggggggcccccccgggaaaaaaaatcggggggggggggggcaaaaaaacccccttttttcaaaaagggggggggaatttcc
cccccccaaggggggcccccccccccccccaaaagggggggggggggccccccccccatttttaacccccccccggttttttaaaatataaaaacccttt
tttgggaaaaaggcccccccccccgggggggttttatggggggggggttcccaaaaaaaagggtgggttccccccaaaaaacccttttgggggggggccc
cccctaatacccccaacaaaaaaaaggggggccccccggggggggaaaaaaaaaagacaaaaccccccccggggggggctttgcgccccccacaaatggg
tggcgcccccccggggggccccccaaaaaccccccgtggggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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