XB-CLONE-4352837
Clone Name: IMAGE:7027555
|
Gene:
ccnb2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783996
[+]
taattaatccccccccccccccgaggtattgcagagtctgcgtgatcctgggcagcgagggttttagcttccttgtctttcggttttatcggttggtttt
aaacgtcctattcccgcagatggctactcgtcgggctgccgtttcccgtgaagcagataatattcttgggggtgcaatgaggtccaaagctcaaattaat
accagaagagctgctttgggggagattggcaacaaggtgacagtgcgaggaaaaccaccttcagtcaagccttccattgttgtggcaaaaccatcaaagg
cagcaactaaaggggcaaatgttaagccgaagcctgtacctgtaaaaacagcagtagctgaagctcccccaaaagtgccctcccctctgccaatggatgt
gtctatgaaagaggaagagctgtgccaggccttctcaaatgccttaaccaatgttgaagacattgatgctgatgatggtggaaaccctcagttgtgcagt
gactatgtgatggacatctataactacctaaagcaactagaagtccaacaatctgtgcgcccatgctatcttgaaggaaaagaaattaatgagcgtatga
gagctatcctagttgactggattgttcacgtgcattctaaggttcaacttcttcaggagactttatacatgggggattgccaatcatggatccccttttt
acaacttcagccagatctcccgcaataaagcttccactttaattgggtgtaaacttcccccaacccggttgcttttccaaaatattcaaaacgtattgtt
ccccccccagaaaggttttggcggaacctcttgcgttcttaataccgccga
3' EST: CF783995
[+]tttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
tttttttttttttttttttttttttttttttttttttgggggggggaaaaaatttttaaaaaaccccccccccccccgggggggggcccccaaaaccccc
cccgaaaaaaaaaaaaacccccccaagtaaaaaaaaaaaaaaaagaccccccccccccccccccaaaaaaaaaaaaaaaaagggggccccccccgagggg
ggaaacccaaccccccaaaaaaaggggcgaaaaaattctgggaaaaaaaaaccccccccccgggggggggggggggtcccccttaaaaccgggggcaaaa
aatttccccccccccccccccccctttaaaaaaacgggggggggggggggggggttttcccccccccttccccaacaccgggccaccccccctttttggg
ccccgggaaaaaaagggggggccccccccccccccccccaaaaaaaaattttttccacacaaaaaaaaccccccccccctttttttgggggggggggggg
gcccccccccccccccgaggggggcccccccccccaaaattttggggggggggcccccccccccccccccccccccaaaaaaattccccccccccccccc
aaaaaaaaaaaaaaaaaacccccccccccccccccgcacaaaaaggtaaaaccccccccaaaaaagggacctccctcccccctccccccccgcggggggg
aagaaagaaccgaatgaagagctgtttttcccgcccggcgagaaaaaaaaaaaaagaagcccgcccgccgcccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: