XB-CLONE-4352825
Clone Name: IMAGE:7027549
|
Gene:
rpl17
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784003 [+]
taattaatcccccccccccccccgccctcttcctgcgcggcctgagattctctcggaacaatggtgcgctattctctcgacccggagaaccccacaaaat
catgcaaggcacggggctcaaaccttcgagtgcactttaagaacactcgtgaaactgctcaagctatcaaaggcatgcacatccgtaaggccaccaaata
cctgaaagacgtcaccctgaagaagcagtgcgttcctttccgccgctacaacgggggtgttggaaggtgcgcccagcctcaatcgtgggactggacacag
ggacgttggccaaaaaagagcgcaaacttcctcctgcacatactgaaaaatgctgaaagctatgcagaactcaagggtttggatgttaattcttcgcgta
ttgagtacatccccgtgcattatgcccccagatatgcccaccgcgctacctaccggtgcccctggcccgttactattccttcacattgacatcgtccatg
accccccttaaaacgtggtattctccctgaaaaacggaatcctcaccatgttattactatgccttgaaccccccgcaaaaccattattttatttcaagaa
taacccgtatttttccttttcacaaaaggaccctccctgctgtgcatattgtcgacccagtatcctccttatttcgcgacccctccacgcgatgttcaat
ttccacatctagtatttccacacactgttacacactcttattccccccttacaatagggatccggcgcgagtcaatatatcccctgtagttggtcgcgct
ccccctccctactttaattctatctgacaccagcctcatagtttatatcacatataatatagctcctgtatctgcatattaccactataatcgtattgat
atgcgatgagaatcacttatcctctatgcgacactcgtctagtgtgaccgtcgcccccnccctctnctcctgtatatttcgccgcacccccccactaccc
tactatatatctctcgctcggataacacacaatcggccgtttgccccctatatatatgttatcactcacgcctccttgtagtccgctttcgttgatccat
gtcctcccagtatatatgatattttgctcgccggtatacgcgcatgttattacgcgcc
3' EST: CF784002 [+]tttttttttttttttcattttttttttttttttttttttttttttttttttaacagttttttgggactttatttctgtacaggttgtatttattcccgag
ccatgagtttctgctttttcagcttcttctgagagatctttttcttttgagcgacttcctcctcaggttttggtacgatctgttccttctcagtaaggat
caattcgatgtggcatggggagctcatatagggattgataccaccatgagcacggtaggtacccctgcgcatcttgggggccttattcacctggatgtgc
tcaatgaccaaagaatcaacatccaaacccctgacttctgcattgctttcagcatttttcaatatgtgcaggaggaagtttgcgctcttttttggccaac
gtccctgtgtccagtcccactgcttagcctgggcacaccttccaacacccccgttgtagcggcggaaaggaacacactgcttcttcagggggacttcttt
cagggatttggtggccttacggaagtgcatgccttttgataacctggaaagtttcacgagtgtttcttaaagggccctccaaggtttaaagccccctgcc
cctggaaaaatttttgggggggttcccccggggccaagaaaaaaacggcaccctttgtcccccaaaaaaaaaccccgggcccccccaaataaaaaaggcc
ctggggggggggcggggggggttttttattttaaccccccagggcctatagtaggtagaaacgccgctcctggcgaattttggtgtttctgtccacccac
accctatagggttctcgccgccatggtgaatttcgtgacaggctggggaaacatcggttgtacgggggaaactaaactaatgcaatactgctggcatact
tatttaacactattcaattaagcgcgcgacaaaacctcttaaaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: