XB-CLONE-4352819
Clone Name: IMAGE:7027547
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Gene:
ocm4.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784007 [+]
taattaatcccccccccccccgacatctgtcacaggaagactgcttgtatctcttcactccagctacagaggacttcggactgaccatggcattcggtgg
tatcctgagtgatgctgacttctctgctgctctgcagaactgccaagctcctgaatccttcaacttcaaaactttctttgcccagtctggcctgagcagc
aagtccgacaatgatgtgaggaacgtctttgtacatccctgaccatacaggagcggctttactgatgaagacgaactgacactgttcctttggagcttca
acgcatgtgccttggctttaactgatgctagataccaatgccttccttgctatctggtgcctctttacgctgtatggcaaatcccggacctgattcctct
tcagtcttctggccgataaactcattttttatttccacccggttttttcccattccccttccccaatatgtacctttgcccttttccaacctttcgtacg
gatcataaaccgcacatatatgccttttacctaccgtgattttggcatgcttataatctccgagtatattccccaccccaacacattatttatttatatt
atatacacacgttttatcttttctatctcgcccgtcaccggtgccattttataccccgtttcccattttatccccccgcgcccagtttacctcccaacgt
ttttcccctctcacccctatttgccgcgccttttattttcctcttcacatatcgcctccttttcctcctaccataatattgtctgcccaccccgtgtttt
tgcttaatttgtggcctatcctatttacctaattctatttgtgcccctaactcggactaattccccaggtgctaagagttccccctcttttttattctcc
ccccccaccacttctatccggcccgcgatcgcctcgccgatccctttatgtataccaccccgcgtcgccctttggtattttccacttatattatttatct
ctcgctgacccatatttagccg
3' EST: CF784006 [+]tttttttttttttttagattccaaaaatgatttattgagagcaatttgtttcttgtatgcctgcctcagtccacaggaaactgaagcaagaattgcaagt
tcatggatttgtaaaagtctcaacagtgtaaacaattgacaacgtgattagtataaaaatgagaataaatatgttggtttaacgaaggggcccttggata
aactgtagacaagggtaaccatgcttctctggtgaattaataaatggatcccaccaccgcatgatgacatcacaacttttctcatgaggagaaatattga
agaggcatctgtgttccgctaaaccaggtttcacttggatcagtttaatgcagaaaaccaaattcatcttttctgatgtttcgcatattccagtttttta
aaaaattagtttttcaccaaagcctggaactcatcaactccaattttgccatcaccatcagagtcaccagcattcaggaaggccttggtctcagcatcag
ttaaagccctggcacctgcgttgaagttctggaggaacagtttcagttcatcttcctcaatgaagccgctcctgtcctggtcaaggatggcaaagacgtt
cctcacatcattgtcggacttgctgctcaagccagactggggcaagaaagttttgaagttgaaggattcaggagcttggcagttctgcagagcagcagag
acgtcagcatcactcaggataccacccgaatgccatggttcagtccgcaagttcctctgttaactggagtgaaagagatacaagcagtctttcctgttga
aaaaatgtcgggggggggggggggggaataaatttat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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