XB-CLONE-4352816
Clone Name: IMAGE:7027546
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Gene:
psmd7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077668
[+]
CGAGGTTCCAACGGAAGTTGTGGTAGCTGTGTGAAGTGTGTGTGTTCCTGCCGGTGTTTGATTGTGTGTATCATCGAAGCCGGGAGCGATGCCTGAACTG
GCGGTAGATAGAGTTGTGGTTCACCCCTTGGTGCTGCTTAGCGTGGTTGATCATTTTAACAGAATTGGAAAGGTCGGAAACCAAAAGCGAGTTGTCGGTG
TCTTGCTGGGCTCCTGGCACAAGAAAATCTTAGATGTCTCCAACAGTTTTGCAGTTCCATTTGATGAAGATGATAAAGATGATTCTGTCTGGTTTCTGGA
TCACGACTATCTGGAGAACATGTATGGGATGTTCAAAAAGGTCAATGCTCGTGAACGGATAGTGGGCTGGTACCATACTGGGCCGAAGCTTCATAAAAAT
GACATCGCCATTAATGAACTGATGAAAAGATATTGCCCAAATTCGGTGCTGGTTATAATTGATGTAAAGCCAAAAGATCTGGGCCTTCCCACTGAGGCGT
ACATTTCTGTGGAAGAAGTTCATGATGATGGCACACCAACTTCAAAGACTTTTGAACATGTCACCAGTGAGATTGGTGCAGAAGAGGCAGAAGAAGTTGG
AGTGGAACATTTGTTAAGAGACATCAAAGATACTACAGTGGGGACCTTGTCGCAACGCATTACAAACCAGGTGCATGGACTGAAGGGACTGAACTCCAAA
TTGCTTGACATCAGGAGCTACCTGGAGAAAGTGTCCTCAGGCAAACTACCCATCAATCACCAGATCATCTATCAGCTGCAGGATGTCTTCAACCTACTGC
CAGATGTCAACCTCCAAGAGTTTGTTAAAGCCTTCTACTTAAAGACTAATGACCAGATGCTTGTGGTCTATCTAGCCTCACTGATCCGCTCTGTGGTAGC
CCTTCATAATCTTATCAATAATAAGATTGCCAATAGGGATGCGGAAAAGAAAGAAGGCCAGGAAAAAGAGGAAAGCAAGAAGGAGAGGAAAGATGAAAAG
GAAAAAGAGAAGAGTGAAGCAAAAAAAAAAAAAAAA
5' EST: CF784009
[+]taattaatccccccccccccccgaggttccaacggaagttgtggtagctgtgtgaagtgtgtgtgttcctgccggtgtttgattgtgtgtatcatcgaag
ccgggagcgatgcctgaactggcggtagatagagttgtggttcaccccttggtgctgcttagcgtggttgatcattttaacagaattggaaaggtcggaa
accaaaagcgagttgtcggtgtcttgctgggctcctggcacaagaaaatcttagatgtctccaacagttttgcagttccatttgatgaagatgataaaga
tgattctgtctggtttctggatcacgactatctggagaacatgtatgggatgttcaaaaaggtcaatgctcgtgaacggatagtgggctggtaccatact
gggccgaagcttcataaaaatgacatcgccattaatgaactgatgaaaagatattgcccaaattcggtgctggttataattgatgtaaagccaaaagatc
tgggccttcccactgaggcgtacatttctgtggaagaagttcatgatgatggcacaccaacttcaaagacttttgaacatgtcaccagtgagattggtgc
ataagaggcagaagaagttggagtggaacatttgttaagagacatcaaagatactacagtggggaccttgtcgcaacgcatttacaaaccaggtgcatgg
gactgaaaggacttgacttccaaattgccttgacattcaggagctaccctgggagaaaagtgttcctccaggcaaactaaccccatccattcacccgaaa
tcattcctatcaaccttggcgggaatggtctttcccacc
3' EST: CF784008
[+]tttttttttttttttgcttcactcttctctttttccttttcatctttcctctccttcttgctttcctctttttcctggccttctttcttttccgcatccc
tattggcaatcttattattgataagattatgaagggctaccacagagcggatcagtgaggctagatagaccacaagcatctggtcattagtctttaagta
gaaggctttaacaaactcttggaggttgacatctggcagtaggttgaagacatcctgcagctgatagatgatctggtgattgatgggtagtttgcctgag
gacactttctccaggtagctcctgatgtcaagcaatttggagttcagtcccttcagtccatgcacctggtttgtaatgcgttgcgacaaggtccccactg
tagtatctttgatgtctcttaacaaatgttccactccaacttcttctgcctcttctgcaccaatctcactggtgacatgttcaaaagtctttgaagttgg
tgtgccatcatcatgaacttcttccacagaaatgtacgcctcagtgggaaagcccagatcttttggctttacatcaattataaaccgcaccgaaatttgg
gcaatatctttttcatcagttcattaaaggcgatgtcaatttttaagaaagcttcggcccaataatgggaaccagcccactattccgttcccgaacattg
aaccttttttaaaaaatccccataattgttcctccaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: