XB-CLONE-4352810
Clone Name: IMAGE:7027544
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784013
[+]
taattaatcccccccccccccggcggaaggaaaacaaacgcgcacgctggctagagtaagcaagtcgtagcgccaagaatgagatggtaacaagaaacaa
aaagcaaaataaagtcggcacctaccttaagaccgagtgactctgacaggaaatactgaaagtcctgttgctatcataagtgcaggtattgcacttgctc
ctgtggcagctttgtaaacactgggtgcttcacaatgggctatcgtaaagcagaacagccggtggccaccttgccaacccccatgcaatactgctttgcc
cggttaaattttttttccttcaggaaggagatccagcaacatgcccttttcctcctttccgaatggaaaaattcaacccttttttggcccataacgtctt
ttcctccgcccctatccctttataccccgtctccagtttctttttcccgtgtgcccccctgtgattcccgcgtctcgagaaatcctcccgggccgttttg
ttgcgctctgcgcccaattatacgcaaattgttgggggagttgtggggaccaccgagaaaatttccacaatatttttctaaccgcacgttatttttttca
accgtcctttgtctgttttcccccccgccaccacgactttaccacaaaaattacgcgccgccctgttatttccactatccatatggagaaaataatttac
atccctctatttatttttaccacccttataattgtttgtttttaaccccctttccgctcaattcttcgcttatatatataccaccattaatttttttttt
taatgcatactagtgtcgctaacctttttttgccctactcagaataataaatctgcatcaatcttcccccccggcgcaaatnatctatcgtggtgtatat
tcccgctcatttcttcccgctgctcccgcgctacttttccgntatgtgtattaacagagcgcaaagcttacgtattcttttttcccacgccacgacgtta
taagtaccgtcgcttattttatccccaatataatggagatatgcctgacattttatacacacgcatatgttttttctattacgagggagtgaatagcgac
gcatattatatttctctcctcacgctcccggaaaggacctatataagataccaccataacggccccgcccgctcacatgttttatactactattttnttt
accaccccacgggagcgcccatcttgtttc
3' EST: CF784012
[+]ttttttttttttttcactttgaaccaaggatttatatcgccatagtaaatggtaatattaactcaaaagcaaaattattgcagtcaatataaagtcttaa
gtcagctgtccatctcttctaatatttctgttttagttttaggatgcttattaacttcttcaggttctatgtttatttcagaaagatcaatctcaggtaa
aggagtcctccagtaattaaatgaattgaattctttgaagtcatcatcactctggcatttgtactctttgcttttgctctccatcaccctttgcttctta
catggaagtgatggctgggaatctggaacattttcaggactcttattacaacttggagactgtatcacgtccaagcaagcatccacctcgagctttcttt
tcctcctgggagtggaagggctgttactgggctcatactgaaaagaggaaatttcccatgccagcattttgcatgggggtggcaaggtggccacctggtg
ctctgctttacgcttagccatttgcagcacccagtgttacaagctgccacaggagcaagtgcaatacctgcactatgatagcaacaggactttcagtatt
tcctgtcagagtcactcggtcttaaggtaggtccaactttattttgctttttgtttcttgttaccatctcattcttggcgctacgacttgcttactctan
ccagcgtggcgcgtttgtttttccttccccccggggggggggggggaataattaactccaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: