XB-CLONE-4352796
Clone Name: IMAGE:7027539
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Gene:
phip
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784022
[+]
taattaatccccccccccccccagctaccccaacatgtcctctctcaacaaccagcacagcaccgagctcaaatccgagctgtatttccttattgccaga
tttctggaagatggaccctgccagcaagcagcacaggttctgatccgagaagtggaggagaaggagttgctgccgaaaagggtagactggacggggaaag
aacacgccaggacttaccagaatttggtgaaatattatcgacacttggcacctgaccatttactgcaaatatgtcaaaggataggcctccttctggaaac
acaagttcctcatagtgtcttatgggtacagacacttttgggcgctggacgacacatctctgccgctcccaaactaaactttttcccacgttgtgtccac
cgcattcctttccgctctttttccctgcggaatgcctccatatccccccataaattatggaattttttccagttaccctaaaccttttttaagtaaaaca
cctgaatgggaaatttccactctgttataccctctcactttacgccgcttactttcaataatgaaccttgtccctatacaaatttttttccggaatttct
ccctttttttttttcccctcgcatttacctatatcccatttttgcccaaggcccccactttaccctcttaactcggactccccccaataattgttttttt
cgttttctgcgcactcagtgttgatctatgttggcaagggcttcgctcacccccgcgtattacactctttagccgcgcgtggctctcgctatattaccca
tctttttttactcacacctcccccctatttataccacaccccccaaatatccacaccttttgacccccccttccattttccgtttttccccccccccctc
tctcattt
3' EST: CF784021
[+]tttttttttttttttccttcttgttctgcttttgtttaaacttatcagaattttcttcatcttctgagctgctttctgctttctttgttctgtgtttcag
tattttctttggtggctgcaggttgattccagcatctgcagtccagtctgaataatcacttgaatagtcacttgaactaccatcactattccattcttgt
ttttcatcctcttcagatgttgccccactagcagcaactgcttcgctctcgacataggcttcatctgatgagctattctcaacatcttcagtaggcactg
cttgttcttcaggagcagctcgagtgcggtagttgtatgaattggacgactgccttttaaggtcctccatgtccagcactaattcccgggagtggttctt
ggaatatgaaactttgctttctcctccaacaagtattggtaagggtttctccttttttgtcccctgccgctccacatacaatattccaaattctctttct
ttgcagattttccccttccttcccggttaacccttaaaacccccccctgagcaaggttctttggaaaaaaaccacctttttttttgcgcccaccgccatt
ataaaaaataccccctttcccggacggagcgaattttctcacatacgccttgggaaaggcacatatccctcttagaatatcatcggccccccaaaaaaat
tacatccaattttttttgtaaactcttcccgcccctttttttcccattttgttttctgaattggggttaaaatttgcctcccttttttttttccacccac
tatcttctcacccctgttgtttatcttgttgcatatgtgtcctctctctccataaaattattctcatcaaatatggttatatatcattcaaccatttctc
tctaaggcatgtttattcctcatcgataagtagaccctaatttataaatttttcatcaccccttctcatattaaaccccaccgatatacagtttatgtac
acgccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: