XB-CLONE-4352783
Clone Name: IMAGE:7027534
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Gene:
pclaf
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077667
[+]
GAGAGTGAGCTAGAGTGTCAGCGTGACGGCAGTGTTAGGGAAGATGGTGCGCACTAAGGCCGATTCCGCGGGCTCTTCTGCCTCGTCAGGCAGCTACAGG
AAGGCTGTTGCTGCCAGAGCACCAAGGAAAACATTTGGGAGTAGTTCCAGTGGTTCAAACCATGTGACTTCACCAACTGGAAAAAAATCTGAAAGTAAAT
ATGCAGGAGGAAATCCTGTGTGTGTCAGGCCAACTCCTACCTGGCAGAAAGGTATAGGAGAGTTCTTTGGATCACCATCCACAAGTCAGCCTGAAAAGGA
AAACAGAATTCCCTCTGATGATGAGGAAGCTGGTGGCAGTGGTGCAGGAAAAGCACCCAGAAAGTCCCGTCCTCTGCCACCAGATCCTTCAGAAGAAGCT
GCTGACTCAGATGATGAGTGAGACTATGATCTGTACTTCCAAACAGGACTTCAGTTTTGTGTTGTATATTTGTAAATAAAACTTATAGTCTAGGAAAAAA
AAAAAAAAAA
5' EST: CF784029
[+]taattaatcccccccccccccgagagtgagctagagtgtcagcgtgacggcagtgttagggaagatggtgcgcactaaggccgattccgcgggctcttct
gcctcgtcaggcagctacaggaaggctgttgctgccagagcaccaaggaaaacatttgggagtagttccagtggttcaaaccatgtgacttcaccaactg
gaaaaaaatctgaaagtaaatatgcaggaggaaatcctgtgtgtgtcaggccaactcctacctggcagaaaggtataggagagttctttggatcaccatc
cacaagtcagcctgaaaaggaaaacagaattccctctgatgatgaggaagctggtggcagtggtgcaggaaaagcacccagaaagtcccgtcctctgcca
ccagatccttcagaagaagctgctgactcagatgatgagtgagactatgatctgtacttccaaacaggacttcaattttgtgttgtatatttgtaaataa
aacttatgggctaagaaaaaaaaaaaaaaagggatccgcctatttctagaagcggccgcccacccccagggggaccttaggccgtgggccccaacttgtt
ttattgcgcctttataatgggttacccaataaagcgaagcagctctcacaaatttttcccaaataaaagaatattttatctcccgggccactccaattgg
gggattttgttcccaacctccatagaagggatccttataccagggcgggggtacgggaaaaataaattctgtggggaaacaaccttggggctggaaagag
acaccctgaaaaaaagaaaacctttgtgttaa
3' EST: CF784030
[+]tttttttttttttttcctagactataagttttatttacaaatatacaacacaaaactgaagtcctgtttggaagtacagatcatagtctcactcatcatc
tgagtcagcagcttcttctgaaggatctggtggcagaggacgggactttctgggtgcttttcctgcaccactgccaccagcttcctcatcatcagaggga
attctgttttccttttcaggctgacttgtggatggtgatccaaagaactctcctatacctttctgccaggtaggagttggcctgacacacacaggatttc
ctcctgcatatttactttcaaatttttttccagttggtgaagtcacatggttttgaaccactnggaactactcccaaatgttttcccttggtgctctggc
agcaacagccttcctgtagctgcctgacgaggcagaaaaaccccgcggaaatcggcccttattgcgcaccatctttccctaacacttgccgtccccctga
acactctagcttccctctcggggggggggggggattacttttcctcggggtgcaaaaagaactattatccttgtattccgagccctggataaaaccccag
gtgcctttttggatcttgggaagtgcaccccttgtgtgataaaaagggccaagggggaactttattccttaaagggtcccccgtaaatctgttatgtgct
tgactccctcgaggcgtctgctattaatttggagcccccgtcttcctatactaaaccccagggggggggggggcttatataacctcttaaagagcgggaa
ccttaagttatccctcttgggggcgggggaaaaccccccatgtcccagaagcaactgtatatctagcgtg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: