XB-CLONE-4352771
Clone Name: IMAGE:7027529
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Gene:
pdcd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784037 [+]
taattaatcccccccccccccgaatgctgtcattgcgcagcttaggaacatggtgaatttgtatatatgacacgtgttagctgctcagaaatggcaaccg
ctagtcaggttacagaactcggttttctgcaggagacagaagcgtggcgactgcgcagccctcagttccccagcaaagtcggggggcgacctgcttggct
gggggaagttggcgtgccagaccctgcggctttgcagtgcggactgtgtgggaagccattggcatttctcttgcaagtgtacgcaccttgtcctggcagt
ttccacaggactattttcattttttgctgccgcaatggtagttgccacagagaatcagagacccggtgttttaaagtttatcggaatcagctgcccagga
aagaacgacacctattcctacggccctcctccagaaacaggctcacctgatggagtaggctacattacctttgagtttgaaatgtggccttcgtctttgc
aaagtctgtggttggtttggggcccaaaaacatgctcaaagtgtcacaaaagtttaattactgcagtaaagagcatcaactgatggactggaaattccgg
cacaaaaaagtatgcaatgaaaagtttgatgtatctagtgttacagtttggagatcatggggtttctgtttcccggaaattgaaaatttgttacttgaac
cctgaaggatatgggagggttgaatggcaaaagccgaaagatgaagaaggatgaaaacagttggaccaaaccttgataccccaaatttt
3' EST: CF784036 [+]accactggccatcaacccaatttgtttgtatagctaacttgcgtgtctcttagagtatagacaaaagcaggtgaacgtgtacaatatgtggattactgga
cactagactctttctaagaacattcaaatgaaagttgtttaacccttacagagctattctgtgggtcctattttaaggatgtaaatgtcacttcatcctg
ttaagctttatgaagttattaaacatggtttgttataatatgcacaagcatacatttaatttcaagcatgcaacagcatacactaagaatctagtgtatc
aacagtgtttacattgttataaattgcaagtaatcaaaaccttattagcctagtaagatttttttcttacattcatgtttttggatggaaatgaatttac
catacaccgttatccacagcagaagatacaatctcagaaaagattttagtgacaaccatatacttttcttatcatataaagaaaactacaaggataaagc
catatacatatacattttcacagtgtgtcaccagcaatatcctgtttccataaaaactctgttgcatatggtttctctccactgcagttggcagcacaag
taaatacagctagaaccccccagtcaatactttctccaaggctgtccacttttagatgattcagaagttgtggcataacctgaaattcaaattgtctttt
cattccacaggaacagtctggaatatctttctcagaaggaatatgcctgtgtgaaatccatagtggatcactacctctgcagtatctcaaaaactgctct
ggttcccaagca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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