XB-CLONE-4352756
Clone Name: IMAGE:7027523
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Gene:
pef1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784046
[+]
taattaatcccccccccccccgacaggcaaaatggcgagttatccgtacggccagggatatcacggagcagctggacaacctccaggtgcaccccaggca
cactactatagtggacagcagtatgggggaggtgttcagcctggggctggttatgggggaccagcacctggtgcaccttatggccctccatcttctgggg
gagtttatgggcatcctgttcctggtagtgctgcacctggagctcctggaggtccctatggaggacaagcaccagggggaccttatagtgtcccaggttc
aaccccttatggaaaccaacaacatggaccttatggccagggagcacctactggaaacattcctccaggagtggatccagaggctttctcatggtttcag
actgttgattcagaccacagtggctacatttccttgaaggagctaaagcaggcactggtcaattccaactggtcatcgtttaatgatgagacctgcatga
tgatgatgaatatgtttgataagtccaactctgggcggattgatctgtttggattttcagctctatggagattcatccaacaatggagaaacctgttcca
acagtacgacagagacagatctggctccattaatcaaagaaagttgcatcaagctctgtgcccgatgggatatccgctttagtctccgtttgtgcagctt
gtaaatgtccagatatgctccagaggtctgtaccaaccaggccctgcaattggatcgattc
3' EST: CF784045
[+]ttttttttttttttgccaccaaggtctgtgccccctattttaaagctgaaaaagagacagaagaggaaaactaacaatttgaaatctttcactatagatt
aattaataagctaagtagagaatatattataacatacttaacgttaagctaacagtgaactgcttcataagatataccaactttggccacacagacagat
accctttttagccacagttccaactaaactgcactaaaggtaaaaagcatttggtttcaagtaaatagcattctaatattcatgggtctaaatttaggcc
cgattttctaaagatcaatatttcttacctataggtaacaatggttaacattcagtaaaaaaataccgatcatttctgatttaacaaatacatttctgtg
ctggaacaagtcccagatccctgcttacaatttgcatagggaataactgatccagagggatgaacagctaccgctgagctcaaacaccccacagagcaca
atggagtttaactgcttgaggaagtctgctgtactgacacatctggctgctttaattactttaagaactctctggataggaagtgttataactgctttta
aatgctaaggatttggaagactaaacttagggcattggatctagatatacattgctcatgtcagtcttgaccagaaatacctatagttacttgctgtgct
ctattactatatatacgtgagaaatatttaggggtgtaccagagtgagtattattctatcactgtgaaagcatccgatttaataaatacgttctatgtac
aggaaaaaaatac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: