XB-CLONE-4352747
Clone Name: IMAGE:7027519
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Gene:
u2af2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784052 [+]
taattaatcccccccccccccggagcgcgagaagagagtgcaggactggcgctcggacacggcctgccgccgccatcacaataaagcgcggccatcatgt
ctgacttcgacgagtttgagcggcagctgaatgaaaataagcaagaaagggacaaggaaaatcgtcatcgaaaacgtagtcacagccgttctagaagccg
agaccggaagcgccgcagccgtagcagagacaggcgaggtagagagcagcgaagtggatccagggatcgaaggcgacgcagtcgctcaccaagacatgag
aagaaaaagaagatccgtaaatactgggatgttccacctcctggttttgagcatatcacccctatgcagtataaagccatgcaagctgcgggtcagattc
cagctactgctctccttccaacaatgacaccagatggcttggcagttactcctactcctgtgcctgttgtgggtagtcagatgacaagacaggcccgtcg
actctatgttggaaatattccatttggaatcactgaggaagcaatgatggatttctttaatgcccagatgcgccttggtggattgactcaagcgcctgga
aatcctgtactggctgttcagatcaatcaagacaaaaaactttgccttcttggagtttcgttctgttgatgaaacacacaggcaatggcttttgatggga
taatttttcaaggccagtctctcaaaataaagcgtcctcatgattaccagcctttaccagccatgtctgaaaaccccatcgtgtatgtaccaaggggtgg
ttttctaccgtttgtc
3' EST: CF784051 [+]tttttttttttttttctaagatgaaaacattctttaattgagatggcttcaaaaaatccatacactctgtaaagaataaaaagtaaaatacaacaaaatg
tttaatgagcaattttgtctttgcaaatatgaaacggtcacagaaagaaaatcaagaaaagacaacattttactacagtataaccaccactgagattctt
tatggtccttgggggcaagaagggggagtggggaaggggaggttctggggttattttacattgtgtggggggtgtgatggtcaaagaataggtctcaaat
ttaattccttaaaaggattagtgaggtggtcatgatgtttcaaggagggaaaaaattacttttggccataaaaatataaacacaatcagaaaaaaaaaaa
gcctcctgcaaaagaaaaaaataaaataaaatgaaggtccttctgatattcccccagcctctcgatcctagctcatctcagcccgccatttcgcttccat
tctaccagaaatcacggcggtggtaaccatcaggatcacagtattttgtaaccaccactctgttagcaaatttgcgcccagtgagtccttgcatggcttt
ctggcaatcaaagactgacgtaaattctacaaagatcttgccgcatcctggcacttcaactccatccactggacgtggtatttcaattgatttgacagcg
ccatatttgccacactcatccctaacatcctccactatctcctcatactcatcatcatctaacaactcttctgggcagcacatattcattaggcacaaca
cctctgttggatgaacacccatctgcnact
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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