XB-CLONE-4352741
Clone Name: IMAGE:7027517
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Gene:
h1-10
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784056 [+]
taattaatcccccccccccccacacgtatcggagcattgagagagcagcaccatggcaccgcagcccgaaaccgctcccgaagctccgctaactcccgca
cagcaagtcagagcctccccttcaaagtccccaaagaaaagaaagaagaaccagccgggcaggtacagccaactggcggtcgatacgatccgcaaactgg
gggagaggaacggctcgtccctggccaagatctacagcgaagccaggaaggtgccctggttcgaccagcagaacggccgcacctacctcaagtactccat
caaggcgctgctgcacaacaagaccctcactcagacccggggggtcggggccaacggctccttccgactcaacctggagaccctgaaggggatgcagggg
cgcaagaaaccctccgtagccaagcggcccgtcagcaaggcaccccgggcccccaggggccacaccaagagtcacgccaagggccacaaggcgagcccca
agaaggcgggacgtgccagggtagcggccaagagcccaaagaaagcgggacgtgccagggtaccagccaaaagccccaagaaagcgggacgtgccagggt
agcggccaaaagccccaagaaaccccgaacccctcagaaaggcacacgaagcatgaagcgtaggcagcccagagccaggctggtataaagtacaagggac
tatggacactagggctgggctttcccattaataaactttccgcttctcactgactgttcct
3' EST: CF784055 [+]tttttttttttttttctttaaatacaaaacctgtttaataccaaagcattgaaatctacaggaatacaaggagtcaagtgcctggaaagctgctgcccgg
actcctacaagaaagggggctttattaccccacacagcgctgaggagatgctgagagtttagcccagccaataggaaagccagctggcccctccctgtca
gacgcacaaggtaagtgccccaatagaagcgctacagatccctactcggcactgggggtcgggcaagaaacacaagagggacagtcagtgagaagcggaa
ggttatttaatgggaaagcacagccctagtgtccatagtcccttgtactttataccagcctggctctgggctgcctacgcttcatgcttcgtgtgccctt
ctgaggggttcggggtttcttggggcttttggccgctaccctggcacgtcccgccttcttggggcttttggctggtaccctggcacgtcccgctttcttt
gggctcttggccgctaccctggcacgtcccgccttcttggggctcgccttgtggcccttggcgtgactcttggtgtggcccctgggggcccggggtgcct
tgctgacgggccgcttggctacggagggtttcttgcgcccctgcatccccttcagggtctccaggttgagtcggaaggagccgttggccccgaccccccg
ggtctgagtgagggtcttgttgtgcagcagcgccttgatggagtacttgaggtaggtgcggccgttctgctggtcgaaccagggcaccttcctggcttcg
ctgtagatn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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