XB-CLONE-4352729
Clone Name: IMAGE:7027513
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784064 [+]
caccgacttaaatacatcagggttggcttccccggggagtacccagaaaaacttctcctcctccttgcttatcttaggaggggctaggaaaccagtgggg
gcccccatcggtggactaagagcacgctctccgtctccttccccttgatcgctccaacttggaggggtctcactagaagactgggtgggactgtccccgt
aatggcgatgggtctctatgctggccattgcccctttaagggtctgtagataagacttggggggtaacacttcttcccgccccgactcatcaaaagcacg
ggatggggggcccataaactccttccaatcttgggtcctgggaccccctaataaccttcggggagcaattttgcttttggccccccctctttcgtgacat
gggtattcaatgaataatctatctcgcggccgttaatttttctaataacgcgggttccttgcttttccccccaaacacccctttctgttcacccggtgga
ccgctcgtccagccaactcgtcctaccacgtatatgacgcgaattaatagtttttactacctccctatatttcatacatggtctccccttctcctttttc
ccattttaacgttcgcctctacaatgtgcctcgaggctttccctccaccgctttattccccccacgtgcgtttacctcctccctcgtttgtgtgcaaaac
acttctgtgacactctgcttgttgttttgtttacacaaacaccgcgtactttattataacacctacttgaacaacgcattgcgatactatgagattaccc
cccattttttcccacccccgtcttttctaactcatctggccgccttctttgaaacccagccggggtaaatagttctttccccccccctttttttccccgc
ggggtgtttatccaaactgttttccaacaaaaagcgcatctaacacacctatacgttgttccctgtgttatataacgtgcacgtttcatttattccttat
ataccacacacctgcgcaatttttcccccaccccccc
3' EST: CF784063 [+]agccccacacaagatcccccagaggacgcctgcgctcccactgaaccacttgcagtggtggaagcggcggcggatgaggagataggcgctcccgccattc
ccgtgctggcggtggcagcggccggggacatggagggtctggaggaaccaaggccgctaatcgtaaaggaggcccgggtgagtctccgtaaaccctggcc
aaagtgggggggcaaagcaaagtttcaccgatgctattcggggtcccgcgacctagattgggaggagtttatgggccccccatcccctgcttttgatgag
tcggggcgggaagaagtgttaccccccaagtcttatctacagacccttaaaggggcaatggccagcatagagacccatcgccattacggggacagtccca
cccagtcttctagtgagacccctccaagttggagcgatcaaggggaaggagacggagagcgtgctcttagtccaccgatgggggcccccactggtttcct
agcccctcctaagataagcaaggaggaggagaagtttttctgggtactccccggggaagccaaccctgatgtatttaaatccgtgtc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: