XB-CLONE-4352726
Clone Name: IMAGE:7027512
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Gene:
hmgb3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784066 [+]
taattaatccccccccccccccgggggactccacacttagcgaacaatacagagtgaagatggctaaaggtgaccctaagaagccgaaaggcaagatgtc
tgcctatgcatattttgtgcagacatgccgtgaagaacacaaaaagaaaaatcctgaaatccctgtgaactttgcagagttttccaagaagtgctctgag
agatggaagaccatgtcggctaaagagaaatcaaagtttgacgatctggcaaaggcagacaaagttcggtatgacagataaatgaaagactttggacctg
taaagaaaggcaagaagaataaagatccaaatgcccctaaaaggccatcatctggctttttcctcttccgctccaaattccgtccgaaaataaaaatcta
ctatccctggctatttccatcggtggatgtggcaaaaaaaactaagggaaatgtcggaataaacctcaatacatgggttaaaaaccagcctttacaacca
accagcgccatctccaaactcttatgcacggctataaacgttttaacgggcgtccgcctttacatttttgtacaaccggctcgttttatcccctttgtcg
tacactcatatgtcgccgtgcggggctcccacctatgttgtttttatccaccaatagcttttagtccacccacggggttttgaacacatatcatttttct
ctgaaactattgcacgcacattatggcatatacacccccacccgtgtgtatgagagctacctcttattcttataacatcaaatagattataagtaatata
agtatcgctctccttttttatcacccccccctcttttttgtaaaattaaacatttccggaaattatttcntcagagcccggggggtgatatattgtttta
cacccctcctctatgcaccacccgtctcgtatcatcaacaccatttatctccaataaaaaccctctcccaacaccccttagtgtttctacaattttttat
tatcctggggattaaacaacatacctgttttatccccacgcccctctctattataccgcatctcccccgc
3' EST: CF784065 [+]tttttttttttttttccttgagtttagctgccttgttgttgtatggctgcttctcaccatcgttaaggttattccacatctcacctagcttctttgccac
atcaccaatggaaataccagggtttgtagattttatttttggacggaattctgagcagaagaggaaaaagccagatggtggccttttaggggcatttgga
tctttcttcttcttgcctttctttacaggtccaaagtctttcatttctctgtcataccgaactttgtctgcctttgccagatcgtcaaactttgatttct
ctttagccgacatggtcttccatctctcagagcacttcttggaaaactctgcaaagttcacagggatttcaggatttttctttttgtgttcttcacggca
tgtctgcacaaaatatgcataggcagacatcttgcctttcggcttcttagggtcacctttagccatcttcactctgtattgttcgctaagtgtggagtcc
cccggggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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