XB-CLONE-4352720
Clone Name: IMAGE:7027509
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784070 [+]
taattaatcccccccccccccggacagcggcgcctcttctccttctctgagcggcggtgccgggtagttacagtgcggagccatggcggggatgatgtgc
ggcgggaccggggcctctcagccggcggatgcggtagtacaggaaatctgccagaagatcagggaccagttcatagagaaatccgggctcaacaccgacc
tcttcgttcccatttcctacaaaactcagcaggtggccggaaccaacttcttcattaaggcgcacattggggataacaagtgtgtccatctgcgggtcta
taggatgttgccccaccagcaggaggcgctgcgccttgacaaattcctattggacaagaccatggaggaggaggttgtttatttctaaccctgcaacacg
gatcatttctgtagatcgaccgccgatggcacataaggggttaaatgcaattcccgcgggaccatctgcccctgctttgtgcccgctcttctctgcccgg
taaccgccttaactgccattttatatcccgcctttacttctttatatattccttcccaaaaaaatcgcataccttgttttaaaaaaatataaagaaggtt
tcccacaaatttcaaaaagcggctggccaccctcgttgtagctttgacctgtggactccaattttttttatttgcccttttacccccgggtttaaaataa
agggcgcttattctttcccccaatttttctcgtttatcacccacttctttttcttccgcgatgcaaccctctattccgctgagttacgtttctcaaacct
gtcgcttaaagtgagtatcctttttacccgtgcattccgggtatatcggtgttatatccaccctccgctccgcacaatagagcccaatgggggtcgc
3' EST: CF784069 [+]tttttttttttttttcttacagatttctattttttttttatatatatataaaaattaaatggcggatataaaatgacagttaaggctgttaccgggcaga
ttagagggggcacaaagcaggggcagatggtcccgcgggaattgcatttaaccccttatgtgccatcggcggtcgatctacagaaatgatccgtgttgca
gggttagaaataaacaacctcctcctccatggtcttgtccaataggaatttgtcaaggcgcagcgcctcctgctggtggggcaacatcctatagacccgc
agatggacacacttgttatccccaatgtgcgccttaatgaaaaagttggttccggccacctgctgagttttgtaggaaatgggaacgaacaggtcggtgt
tgagcccggatttctctatgaactggtccctgatcttctggcagatttcctgtaccaccgccccctcctgctgaaacgccccggtcccccataacatcaa
ccccgacatgggctcccgcctgttaatacccggcaccgccgtttttaagaaggaaaaaaagcctcctcttgtcaggaggggggggggggaattttatatt
ccctagttccccccccccacccaaattaaatggcaaataacgccgcaataaaaaatactaggccccccgctgcacttagtaaacccggaaccccaataaa
acatctattagatcccgcggtattaacaattcaacagcgtgcccccttttttctctaaccattaaattaaactattaatcccgcatcttacaaatatatt
cgtacgtgccggcgtgtaaactattacgcgcgcccgcggtttttctataaatttcatctccgcctctccttcacccccccccccccatcaaatataaaac
gcgccccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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