XB-CLONE-4352714
Clone Name: IMAGE:7027507
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Gene:
tfg
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080473 [+]
GCCTGACGTTGCGGTGCTGGCAGACAGAAGGAGGAGAAGTGGTATTAGTACTGCCGCCCTCACCCTGTCTCCTTGGCATTTAATCTTACGGCGGTCGGTA
ACCAGCCCGGTATATCTACGGGGGAGCGAACAGCAGCGTCAGCTGCCTAAATCACCACGGAGCCCACAATGAATGGACAACTGGACTTAAGTGGCAAGTT
GATTATAAAAGCTCAGTTGGGAGAAGACATCAGAAGAATTCCAATTCACAATGAAGACATTACCTATGATGAGCTGGTGCTGATGATGCAAAGAGTTTTC
AGAGGAAAGCTCCTTACTAATGATGAGGTGACCATCAAGTACAAAGATGAAGATGGAGATCTTATAACAATATTTGATAGCTCTGATTAGTCGTTTGCCA
TCCAGTGCAGTAGAATATTAAAGCTCACCCTTTTTGTAAGTGGACAGCCAAGACCATTGGAATCTAGTCAAGTAAAGTATCTCCGCCGGGAGTTGATAGA
ACTACGTAACAAAGTTAATCGGTTGCTGGACTGCCTGGAACCACCTGCAGAACCTGGAATACCGTCCAGCCTTCCCGACAATGAAGCTGTGGATGGCAGA
GAAGAGAAACCACCTGCACCAGATTCCTCTGTTAAACCATCTACCCAAGTTATGGCTGCCAGCATGTCTGCTTTTGATCCACTGAAGAACCCAGATGAAA
TCAACAAAAATGTCATGTCTGCATTTGGACTTTCTGAGGAGCAAGTCACTGGACCTCCTAGCGCACCAGTAGAGGAACGTTCCAGCACGCCAGATAGCAT
TGCTTCTTCCTCCTCTGCACATCCACCAGGGACTCAGACTCAGCAGCCATCATACCCAGGAGCCCAGCAGCAAGCAGGACAGATATATCAACAGTATCCA
CAGCCACAGCAGCCTGCTGGTTATCCAGCTCCTCAAGGCCAGCCCCAAGCTCCAGCCCAGCCTCAGCAACCCTACGGCATGCAGTATTCTGGTTACAATC
AGCAAGCTACTCCTCAACCAGCTCAGTATCCAGCTTACAATCAGCAAGCAGCACAGACTCCAGCTCCAGGGTTCCCAGGGCAGCAACAGCAAATGGCTTC
ACAGCCCCCTCAGCAGTACCAGGCTGGGGGATACCCTCCACAGACTTACACTACACAAGCCTCTCAGCCTGCAAACTACAATCCGCCTCCTGTTTCCCAA
CCTGGACTGGCTCCAAGCCAACCAGGGAGCTATCAGCCTAGGGCAGGATTTACACAATCCCCTGGAAGTACTGTAACCCCACCCCCAAGTGGCCCTAACC
CATATGCGCGCAATCGCCTACCATTTGGTCAGGGATACGCTCAGCCAGGACCTGGTTACCGGTAAATGTCACTCTCACTATTACTGCCAAGGCAATGGCT
GCTAGGTCTATATCCATCCCAATAGATTCCAATCTTATGCTTAAATTGTACTGATGTTTCAGACATCAGAGGAAAAGCTTAGTGTGCTTCTCAGCACACT
GTTGCTTTTCACTAAATGTTGTGTAATTTAATTCAAGTAGAGACCAAATTATTCTTTTCTGCTTTTGATAGTACCTGCTCTCTATAGTAGACATTCAAAT
ACAAAAATGTGTTTGACACTAGCCGGCCTTGTCAAAACTATGACACAATGCAAATGGCAACCCCCATTATTTGGCTTGTTTTTAATGATCTGATGTGTTA
AATTAGATCTGTCCTAAAGTTTAAACTAGGTATTATGTACAAATTCTAACACATTAAAGGCTAATAAAACAAAAAAAAAAAAAAAA
5' EST: CF784074 [+]taattaatcccccccccccccgcctgacgttgcggtgctggcagacagaaggaggagaagtggtattagtactgccgccctcaccctgtctccttggcat
ttaatcttacggcggtcggtaaccagcccggtatatctacgggggagcgaacagcagcgtcagctgcctaaatcaccacggagcccacaatgaatggaca
actggacttaagtggcaagttgattataaaagctcagttgggagaagacatcagaagaattccaattcacaatgaagacattacctatgatgagctggtg
ctgatgatgcaaagagttttcagaggaaagctccttactaatgatgaggtgaccatcaagtacaaagatgaagatggagatcttataacaatatttgata
gctctgattagtcgtttgccatccagtgcagtagaatattaaagctcaccctttttgtaagtggacagcccagaccattggaatctagtccagtaaagta
tctccgccgggagttgatagaactacgtaacaaagttaatcggttgctggactgcctggaaccacctgcagaatctggaataccgtctagccttccccac
aatgaacctgtggatggcaaagaagagaaaccacctgccccagattcttctgttaaaccatctaccccagttatggcctgctaccatcgtctgccctttg
gctccaatggaaaaaccctccatgaatattccccccacattgtttattgtcttggcattctgcgactttttttgagggggccaaaggtcacctcggatat
ttttccttaggcggcaccattttttaatacgcaaacgccttcccccaatactaactga
3' EST: CF784073 [+]tttttttttttttttgttttattagcctttaatgtgttagaatttgtacataatacctagtttaaactttaggacagatctaatttaacacatcagatca
ttaaaaacaagccaaataatgggggttgccatttgcattgtgtcatagttttgacaaggccggctagtgtcaaacacatttttgtatttgaatgtctact
atagagagcaggtactatcaaaagcagaaaagaataatttggtctctacttgaattaaattacacaacatttagtgaaaagcaacagtgtgctgagaagc
acactaagcttttcctctgatgtctgaaacatcagtacaatttaagcataagattggaatctattgggatggatatagacctagcagccattgccttggc
agtaatagtgagagtgacatttaccggtaaccaggtcctggctgagcgtatccctgaccaaatggtaggcgattgcgcgcatatgggttagggccacttg
ggggtggggttacagtacttccaggggattgtgtaaatcctgccctangctgatagctccctggttgggcttggagccagtccagggtggggaaacagga
ggccggattgtantttgcaggctgaaaagcttgtgtanttgtaaattctgtgggagggtatccccccaccctggtacctgctgaagggggctgtgaaaac
ccattttctgttgcctggccctggggaacccctggaacctgggaaactggggcccgccttgggctaaattgtaaact
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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