XB-CLONE-4352711
Clone Name: IMAGE:7027506
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784076
[+]
taattaatcccccccccccccgaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcaca
caagccctcagtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcgg
ctaaaccagccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaagttccagcaaaggg
taaaaagggagcaaagggcaaacagacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggagaaacaaaaagtgacgaggcc
cctgcttctgatggaggcgacaaggaatcaaagtctgaataaccttcatcttgtaccgagtcttttatcagtggttcctgtacctcccttgtacaattca
gaggaatatttttatcaactattttgtaaatgcaagttttttagtagtttaattctagaaaacacatttttaaaataggagggaatccccaccatttttt
tgttgtaaaaaaaaattttttttttaaacactttatgaatttggtacattgccccctgtatggtttttaaaaggcctattgaatacttatacccaggtta
aatactaaccacccccaaaaccacctttactgttgggggagttgcaaagccctttgaagcaggtctctaaaaatggttttttttcttaaattcttggtta
accaaaaaacggggcttagttaaacttcctattgaccaagggtttgccccgggttccgaacccaagggcaagtggcccccccaaaacacctggggtaaca
aaaac
3' EST: CF784075
[+]tttttttttttttttctaaagttcagagagaagttgtttcgtagaaaatctagcacccctcgtaccagcatctttgatgcaaataattcaacccatttct
cggccaagaaggagcgaacacgcataggtgccaccttggcaggaaagagatgcaaacacatgaaccgccgtgtcctttttgtttttctccctacttcaca
tcacactcactacacactcaaagaagtttccccagaagagattcacagcacttttacaaagttactgaaaataaaataccactacccagaaaaaaaaaaa
ataaaatggtaacatcacagatagtacacaattctttagagggaaatccgtcaccttgggagatttattagtttaaaagtgtaaattgtacgcaggacaa
catgagtacaaagaacagttaaaacgttaagacattttaatagccgtgatctttgcgccagttgtaccagtatcgggcactgcctgttcgaccggcagcc
tgcatagagtactagccgttttgtaacaaaataaaaaaaacattttagactgctcaaggcttgcactccccacagtaactggtttgtggtgtagtattaa
ctggtatagtattcatagcctttacaaaccatacaggggcaatgtaccaaatcataagtgtttaaaaaaaattttttttacaacaaaaaaatggtgggga
ttccctcctattttaaaaatgtgttttctagaattaaactactaaaaaacttgcatttacaaaatagttgataaaaaatattcctctgaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: