XB-CLONE-4352703
Clone Name: IMAGE:7027503
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Gene:
mapre1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784081
[+]
taattaatcccccccccccccccccccggaggccggttgcagagcctgagaggtgggtaattgactggagcggcgtgtgttcggcggcagtgagaaaatg
gctgttaatgtctattcaacatctgtgactagtgacaacctgagccgccatgatatgctggcatggatcaacgagtccttacagctgaacctaactaaaa
tcgaacagttgtgttcaggcagtgtatattgccagttcatggacatgcttttccctggatcagtcgtgctgaaaaaagtaaaatttcaagcaaaactgga
acatgaatacattcaaaacttcaaggtcctgcaagcgggcttcaaaaaaatgggtgtggataacataactcctgtggacaaactggtaaaaggaaagttc
ctggacaatttttgattctgtggactggttcaaaaaattttttcgatgccaaactatggatggggaaagaattaccacccccgtttgtcaacttcttttc
aggtaaaaggagttcggcccccagtttcccggttcttgggccgcctcccagtttccttgaacacaagttcttaaaaaaaaaccgttcaaggagctcccgg
ggaaaaacccccaacgttcccccccaattaggcacgattttgtcactggcgttcaaaaaaaggaacgttgcccatgttataggttaaaaaaaggtgccct
cccgggagccccaaggggctatttaatncctaaatataattgccctagctttaccctctttgcgcctatttgcccattattcaacacccacctccttgtc
accacacttattatttccacaccccacattatttttccggcccttgggaataaaaatatacttttgttctgatggccacttttttttttaacccggtttt
tacaacccaccccttttttctttgttgttcccgccagtgtctcgaacaaacgattttttgaatcccaccactctcgtctcttttgggaagaacaccaccc
ccgcccttctatttttctt
3' EST: CF784080
[+]tttttttttttttttggcatcaccaaatcccaatttattgtaaaaacagcagggttcctgctccagaggtgaagcctggagaaatggggcacaggggggc
acagcaggtacagaagaagaaggaggcgcaacaggaacataaatacaaggtctcacaatattaaaacaaaggaagagggtgtcggacacaaattcatgtg
aaaagggaaatgctgataactcagaggtttgccactcggtaaatttcatctcattttagtacaggtacatgttaacccccaggctgccgggggggggcac
aatctcaattccatgtgcagacagagcagaagaagagcagcaggattcggcacaaggagcagcccctcgggcacgtccctactcacaagcactacacact
gcaaggagcagcccctcgggttcgtccctactcacaagcactatacactgcaaggagcagcccctctggcacgttcctactcaaaagcactatacactgc
aaggagtagccccttgggcaagtccctcctcacaagcactatacactgcaaggagcagcacctcggcacgtccctactcacaagcactagacactgcaag
gagctgcccacatacacatttactgtatgacttagaaaagcatttagaaaccccttaaggcattggtatatatactgtacaacaccactataacagtctg
accagtaactgaaacttcctcagcccattacacacacttttcctggccacaaaacccatagtatgtcccaatccctaaggcactgtaaaaatgagtggaa
actttggcaaaagtgcac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: