XB-CLONE-4352698
Clone Name: IMAGE:7027501
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Gene:
ubc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784084
[+]
taattaatccccccccccccccggcagttacactttagcaacggtgcagcttggaggtaactggttgagattcgtgcaggaccggatcggcagcaaacat
gcagatatttgtgaaaacgctcactgggaagaccatcacactagaagttgagccaagtgacactattgaaaatgtgaaggctaaaatacaggataaagaa
ggcattcctccagatcaacaaaggttgatctttgctggcaaacagctggaagatggccgaaccttatcagactataacatccaaaaggaatccactctgc
atttggttctccgtttgagaggtgggatgcagatatttgtgaagaccctgactgggaagaccattacccttgaggttgagccaagtgatactattgataa
tgtaaaggcaaaaatccaagacaaggaaggcattccccctgaccagtagagattgatttttgctggcaagcatctggaaaatggtcgcactctttctgac
tacaacattccaaaagaatccaccttgcacttagttcttccccccagaagtgggatgcacatacctgtgcataccccgcccgctccaacaacaatccctt
gaggctgcatccccgaggattcccattcaaatagcgaatgccgacatcccccagattacggaccgttttttccccccccacccataaaaagaagtgatcc
tttcgtctgggaatagcctgcctggaaaaatagggccctaacaaccctcccctggaatttacccccccccccctaaattgaaccgccataccatgcgcca
ttgcggtttcatttccttcccgccgtgaaagcgtggtggaaatcgcacccaatattgttttgtttcccccctaccgcctgctttattgggcgc
3' EST: CF784083
[+]tttttttttttttttggaatgcaatagctttattgaaacaaagtgcaacaagcaagtaatgcagagcagatgcataatgattgttttaaaccccacccct
caaacgcaggaccaggtgcagggtggattctttctgaatgttgtaatcagagagagtgcggccatcttccagctgcttgcctgcaaagatcaatctctgc
tggtcagggggaatgccttccttatcttggatttttgcctttacattctcaattgtgtcactgggctcaacttcaagagtaatggtcttcccagtcaggg
tcttgacaaaaatctgcatcccacctctgagacgaagaaccaagtgcagtgtggattctttctgaatgttgtaatcagagagtgtgcggccctcttccag
ctgctttccggcaaaaatcaatctctgctggtcgggaggaataccttctttgccttgaatttttgcttttaccttttcaacggtgtcacttgggtcaatt
tcccggagtatagattttaccagttaaggtcttttctatttttctggatccctcctttttgaagagttacaaataaagtgcaagggggggattccttttt
taaaatattgaactccagaaaaaggcttcccccccttcttcctaacttgtttagaaaacaaaatataaattttccagttaaaacagaaaggtatttctct
cccctaatttttgaatttttagcctttccccgttttttatgttatctcatttgccttttttttcttatcacgtatgggcttttttttttttatacgcccc
accctcaattatttagcccttctttctcccgccgccaggattaaacataagttccaatgccgtgattcctttatttaaaatgtcttcaccccccaaaaaa
aatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: