XB-CLONE-4352695
Clone Name: IMAGE:7027500
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Gene:
gas1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784086
[+]
taattaatcccccccccccccccccccaccaaagtggaaggctcctatgaggattatcagcagccgtctgggaagcccggagagcaggacggttccatta
aggggtcgcccagtggggcacagactgctgcatggactggctgcttgctgctgggagccattgtgctggcactgcctgtgctatagccggagagatatgg
acacaaactcacttgaagcactgggcctccatcaaaaagccactggccccatgggaccctttacaaaaaagataccaggggtcctttggggctaatcgaa
cagtactaaatgtttccaggactcaggaatgatgctgcccagtataattagaacccctatggatgccatggaggatattgctgcaaatgccagccttaca
ttttgtagttttatgcagacagttgttcctaataactggacatataggttagggttcctgagtgcttggtgataccttaagaatgtactgcttgtattct
accaggtgctgctgaactacaactcctgatccttacctgcatttggagttgaagggtcattaagagctgttgttaatcaacagctggggggctgcaagtt
ggcaaatattccggcctactctttactagcacctttctgctttactggtgcaccagcttgtgatttatattaatacccacgattggtgtccttgtgaatg
aatgttaaatgctttctaaatttccccccgggctattttacggaaatttttggggcccaacccacttctaattggggctaactggggctggtaatacttt
aaccccaaaagtggccccccacagttctcctggggggaaatttaaccccttgggcccatttttctcgt
3' EST: CF784085
[+]tttttttttttttttcaagtttaagaataaactgttattatttcatttataaaaatacaaacgtgtaaggcggtatcttcaagacaacatgtatacattt
ctacaaagtccataaattctctgcaaaaatagctttcacttcaacaaatctataaaatacactgtacatttgaagcacatttctgttttttttataatgt
accttttctgtcaagaaattgtttgcaaacacataaaatttcatctctatattataaataaattattcatcagagaatacaattcatataaaaaaatatg
tacagatgtaaagtcatgagttacaaaggcaatatttaaatacactatttataggtcttgctgtaaccagaaaggatcactttgatactcctattaacaa
tatttatttttctgtatattttatttacagggtattcataaaatgaactttcttaaatggcagtgcacaactgtgtaacccaaggttctcttcggtttat
ctaaggtttaaaagaatgcagatatacccggtgcaaagaaagagtacagttcaactagtaaggccacaactcttttctcatacaaaattacacaaatgtg
atctagataaatgtttcaaggttaaaggcacaatataggggcaaactattacaccacaaatgtgatttttcaactggctataaaggagcagttgtagtac
aaaatagtgttccactaagttctgtggagtgcctaccaatcatgggttagtcattttagcccaactattaactagtttcctttacgcaccaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: