XB-CLONE-4352692
Clone Name: IMAGE:7027499
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784088
[+]
tatcaatccccccccccccccctcttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaaggagaaga
ctcacattaacatcgttgtcattggacacgtagattctggaaagtccacaacaactggacatcttatctacaaatgtggtggtatcgacaagagaaccat
cgaaaagttccagaacgaatctgctgagatgggcaagggctccttcaattacgcctgggtcctgtataaccttaacgccaaacgtgtacatgcgatcatc
agtgacatccccctgtgaaattttgataccacgccatattatgttactcctcgttgaagttccaccacccatatacctcatccagaaatccgattaactg
tttacctcttcatgatcgaccgtgttagcccggattgttttccttcccgcgttgtttacatttccacttccgctttcttttccttttccgtttaacatct
tccgtaataccatgctttttccctttgtattaacccctactgaggaatgaattttgccctcctgaattacctttgcttgtccccaccccctaaacggttt
ttttttatcttgtattcccccctttttcgttggttgtgggcccaaatacggtactcctaataacgcccctttcccgttttttcaacgaaacgaccgttca
cccgccaacttttttgtcccgtcgacccctattatccccgttcctatcacaccaccccttacataaaccccatatttagccgcgccgcactctctattat
taaatctctcccccagtattgtgtgatatgatgttgtcgacataatattgcgttcatatgctaaataccccataactgccataaattataagcacctttt
gagactatctacacctgctcgttattttacccccccccacctcttattttcgcgggtggataatagtagccgaatcccgtattatatatcctcccgcctg
gtcaaataatatctttaaccctattagttttttccccctcgcgcgtttttcccc
3' EST: CF784087
[+]tttttttttttttttgttatcttttaacttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaa
aacactaataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttg
actattaaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgc
ctttctgtgctttctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtc
acggacagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttc
aggaacttgggattgtcttccagtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatcca
acacaggggcatatccagcaccaatctggcctgggtggttcangatgataacctgtgctgtaaaggtaccagctttcattggtgggtcattcttgctgtc
acccagcacgttgccacgacggacgtccttcacagaaacgtttttcacgtttaaagcaacattgttcaccgggcatggcctcgctaaagggcttcatggg
tgcatttcaacagaattaaccttcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: