XB-CLONE-4352677
Clone Name: IMAGE:7027493
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Gene:
gamt
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784098 [+]
taattaatccccccccccccccgctctctcccctgtctactttgaacaaccaaatcaatgtctgaacggatcttcatagagggcgagagctgcaagtccg
cctggcacaatgcaactgctgggtacgatgataccgacactcacctggagatcctgggtaaaccggtaatggagcgatgggagactccatatatgcacag
tctggcttctttagcggccagcaagggtggccgtgtgctggagattggttttggcatggcaattgcagctactaagttggaagagtacaatatcgaggaa
cactggataattgaatgcaatgatggtgtattcaagcggcttcaagaatgggccacaaagcaaccacataagattgtgccattaaaagggctgtgggagg
atgtagtgccaacattacccgacggacactttgatgggatcctttatgacacataccctctttccgaagaaacatggtacacgcaccagtttaactttat
caagggtcacgcttatcggctgcttaaaactggaggagtccccaccttctgcaacctgacctcttggggtgaactattcaagcccaatacaatgacttac
aaaaaaatgttccccgaaaacccaaaatcccatatctagtgggaagcttggtttttaagtctggaaaaaatttaggttcttacctggtaaatggaaccct
tgaataccccccccgagggaattgtgccaaacaattatctcctttatccaaaaaaagttacttcccatccgcaaccttttttacttaatagggcccgtaa
taaacacccctggaaaaaacctcaccctttgggccccccaggtggaaccccttttacacccctctctcacatggg
3' EST: CF784097 [+]tttttttttttttttccaaatatatatggttttattaagcattcgctagcagagtgcttgctgtataaaatggaactttgcaactctgccctgatggcct
taaacagtctatcatattgtggtaggtccagataaatccagtaattattttgtagatagccagtggtgacaattttgattcctgtccctgttagggtcta
ggttcctattaacaaggtaggttctcaagtttctcagaccttaataatggttggtgtgatcatttttttgaaggagtagtatctgcaatcctcggggggt
atcaggtccattacagtagtactaatattctcagacttaaaaccagcatccactagttgtggaatctgagtctcctggaacattttttctatgtcattgt
atttggccttgagtagttcaccccaagatgtcaggttgcagtaggtgaggactcctccaggtttaagcagccgataagcgtgacccttgataaagttaaa
ctggtgcgtgtgccatgtttcttcggaaagagggtatgtgtcataaaggatcccatcaaagtgtccatcgggtaatgttggcactacatcctcccacagc
ccttttaatggcacaatcttatgtggttgctttgtggcccattctttgaaccctttgaatacccctcctttgcctttaattatccaggggttcctcgaat
atttttccccttccaactttaataacctggcaattggccatgccacaaaaccaatttcctccacccacccgggccacccctttggttgggccccgctacc
caaaaagccagaaacgggggaaaaaaaaatggaattccccacctatttattctccctccacctagcgttttatcctcccgcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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