XB-CLONE-4352667
Clone Name: IMAGE:7027489
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Gene:
flot1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784104
[+]
taattaatcccccccccccccggttacggaaaagccgagaggagcttacatctaagagtcaagatggtgttttatacatgcggccctaatgaagctatgg
tggtatcaggtttctgccggagtcctcctgtaatgattgctggcggccgtgtatttgtccttccatgtgttcagcagatacagaggatctccctgaacac
actgacactgaatgttaagagtgagaaggtttacacacgtcatggagtgccaatatctgtgactggtattgctcaagtaaagatccaggggcaaaataag
gaaatgcttgcagctgcatgccagatgtttcttggtaagactgagaatgaagtggcacaaatatccctcgaaaccttggagggacaccagcgtgccatca
tggctcacatgactgttgaggaaatctacaaagacagacagaaattttcagaacaagtttttaaagtggcttcttctgaccttgtgaatatggggattag
tgtggttagttacacactaaaagatatacatgatgatcaggattatctgcactctctgggaaaggcacggacagctcaggtgcagaaggatgctaaaatt
ggagaggctgtggctaaaaaagatgctggcattaaaagagcccaggcaatgcaagaaaaaggtttctgccccatatgtgaatgaaaatagaaatgggcaa
aagctcagaagagacttttgagctgaaagaaagcagcatatgaatcttgaggtttacacccaggaaaagcaaaaaagtgaacttgggctttatcaaccta
caaggtagcgaa
3' EST: CF784103
[+]tttttttttttttttataggcacaccataacagtttttattgaaatagaagttcttattcataaagatatattgtgttataatgtaacaatcttactgag
ctacaggaacaagattcacagtgacaacacaaaatgtaaaggtaataatgtagattttgcacacaataaaacatttaagggcagatttatcaaacattaa
gagctcttccccccccacccccactggattttgactagtaaaaaatcctgcatttttcctgcagtttgagtttaattaaaaaacaaaacaaaaccctgga
gcaattctcacttctttttagcaacattactttgttcaaaattcacagatctgtgtatccaggcaaaataaaaaaaaaagtctgattcttcattttaagc
aattagtgagaaaagccaagtgttttagctttagaaatatagttaatgcccaaaaactaaagtgttttagctgctgaaaaaaaacatgaaaaggttgata
tttgataaatcttccccatagatggtgtggggtgaaatgtctgctccagttctaacccactgcaccagaacaacaggcatcattaaactaatgtagtctt
tatgtgaaagggcaattcttttggtatgggttactagacccggtgcaaatttcaagcctgttattacattaatcacttaaaactaaaatgaaacaattta
atgactgggcaaatctgaatagccatgtttaagctttgatgaaggagaaaaaaaaaggagtagggcaattgatatgaaatatggtcataatcatttatat
ggaatggtgggtattaatcccaaataagccctgtgaatatttta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: