XB-CLONE-4352659
Clone Name: IMAGE:7027486
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Gene:
glul
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784108
[+]
taattaatccccccccccccgctcagtgggtttgtcaacaacttgctgagggaccagagggccagtgctgcttcccttgctaataagatcagccgccgcc
atgtccgtctcccacagttcgagactcaacaagggggtgagggaacagtacatgaaactgccccaaggagggaaggtccaggtcacctacgtctggatcg
acggcaccggggagggagtgaggtgcaagacccggactctggatcaggaacccaaaaccatagacgaaatcccagaatggaacttcgatggatccagtac
gtaccaagcagaaggttcaaacagtgacatgtatctcgtcccagtccagatgttcagagaccccttctgcctggaccccaataagctggttatgtgtgaa
gtcttaaaatacaaccgcaagtctgcagagaccaacctccgacacacgtgtaagaagatcatggaaatggtgactgaccatcgcccatggtttggaatgg
aacaggaatacaccctgctgggcattaacgggcacccgtatggttggccaaagaacggcttccctgggccacaaggtccctattactgcggcgtgggggc
ggataaagtggtacgggcggaacatcgtgggaggcgcactacaagggggtgtctattccccgggcattcaagatctggtggcaccaacgccggaaagtca
tgcccctccccagggggaattcccaggtgggaaacgttgccaaagggcttccaacatggggggaaccccctaatgggatgggcccagg
3' EST: CF784109
[+]gatgctggcgccccggttggcgactccggccgagaactcgtggatactggacgtctcgtgctgcccggtgagtctccgggagttgtccttcccgccccgg
gggtcatacacgcagatgtggtagttgtgcctcttcccgagcttctcgatggcatcttctatgtgtttgagtcccccttcgaccctcatgctctctgtac
tgtagttggtatggcaaccagcgccgttccagtttccggtcatgggcttggggtccagcgtggccaccaccccgaagtcctcgcacacccggtggaggat
gaacctggccatccataggtggtcccccatgtcgatgccttcgcacggtcccacctggaactcccactgggagggcatgacttccgcgttggtgcccaca
gatcttgatgccggcgtatagacacgccttgtagtgcgcctccacgatgtcccgcccgtaccccttatccgcccccccgccgcagtaataggggaccttg
tggcccagggaagccgttctctggccaaccatacgggtgcccgtttaatgcccagcagggtggtattccctgttccattccaaaccatgggcgaatggtc
agtccccatttttccatgattcttcttacaccttgtgttcggaaggttggtcttctgcagaccttgcggggtgttatttttaagaacttcacacattaac
caagctttattgggcggtccaaggcagaaaggggtcttcttaaacatcctgggactgggacaaagaaaacatggtcacctggtttggaccctttctgggt
tttgggaacgttactgggaatccattcgaaatttccatttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: