XB-CLONE-4352651
Clone Name: IMAGE:7027482
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784113 [+]
taattaatcccccccccccccttctggtggcattgaaagatctttcttgattatttaaggagaaccagtgtggaaataacagaactaaagtaacatgctc
tgatgtgggtgggatgaaagctgcaccaaggtccagcgagctgtaaaatacggtcaaatcgagtgccattttatttgttcaaaattaattgaattattgg
aatgcacaaattttttttaatatgcaaataaaaatgataaaacattaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaataaaaaaaaaaaaaaaaaaaaaaaaggggttccctttttttttagggccccccccccccggggaaatttggccgggcccccttt
tttttttggccccttaaaagggttcaaaaaaaaaaaaaatgccccaatttttccaaaaaaaaggtttttttccccggcctccaagggggggtttgcccca
cccccaaaagtgttttttaacggggggggggggggaatatatttgcggcctgccccgggggctaaaaaaacccttcaaaaaaaaaaattggggtagggac
ctcttcggggggggaaaaaaaaacaccctgggagaatgtgtgttcaaattaggggggggggaaaaacccccgagttcccctcttaggggggaaaaatttt
ttacaaaacccgcgcccccctattatgccccccccccccgggggggggaaagattcccccggggccccccccgtgggggggagaaattttgtcaatgaat
tncgccccccaattatttgtcccccc
3' EST: CF784114 [+]tttttttttttttttcctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttnaaagg
gtttccccttttttttggcaaaaaaaaaaaaattttggcctccccaaattttttttttttttaaaaaaaaaaagggggcccccttttcccccttttttac
cccccccggggccttgggggcctttttccccccccccccaaaaaaaattttttttttttttttttttttccccccggggcccccctaaaaaaaaaaaaaa
aattttttccccccccccaagggggggggggggggttttttttcccggggggaaaaaaaaaaaattttttttttttcgcccccacaaaaacccaggggcc
ttttgccccgggaggggcccccttgggaaaaaaaggacaagatgggttttttttctgggggcccccaaaccgtgtggggttaaaacacaaggggtttttt
tttttttggccccctttttaaaaaaaacaggggggggggggcctttataaccttttggggggggatttatcacccccttggggggggggagaaactcttt
ttatatgacacctttccccccccccccgaaggtggattagccccccgggttttttttttggggggttaaaaaacccgcgggggggggcctcccccccggt
tttttttgggtttttataaaaacacccctttcctttttaagaaacccctccccgcaaaaacccccctccccccctttttttttgttaagaggggggggaa
tttttttcaacacatttttggtaaaccn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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