XB-CLONE-4352645
Clone Name: IMAGE:7027480
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Gene:
gid8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784117 [+]
taattaatcccccccccccccgccgtcagctgacccgggcagagaactcgcagcgccggtgacgcagccgccggaagtagtgacaatgagttacacagag
aagccagaggagattacaaaagaagaatggatggaaaaactgaacaacttacatatccaaagagcagacatgaaccgcctaatcatgaactaccttgtta
cagagggattcaaagaggcggcagagaagttccggatggagtctggaatcgaacccagcgttgatctggagagcctggatgaaagaataaagatccgaga
gatgattctgaagggccagattcaggaagccattgctctgatcaattccctacaccctgagctgttggataccaaccgttacttgtactttcacttgcag
caacagcaccttattgagctgatccggcaaagagagacagaggcagctttggagtttgcccagacccaactggcagagcagggagaggagagccgcgagt
gcctgactgagatggagcggacgctggcactgttggcattcgataacccagaggattctccctttggcgacttgctcaacatgatgcaaaggcaaaaggt
atggaagcgaagtcaacccaagccgtcttggattatgagaacccgcgagtccacccctaaaactgggaaaactggctcaagctgctccctctggggctca
aaaagaactggaaccagaaaaaagttaatacccccagaatggctgaacctcagccaaagggacaggggaaaaagccccaagtc
3' EST: CF784118 [+]tttttttttttttttacgtctattaaactgtattacatttgccgcggggcagaaagcttgcacgactggttaacacgctcactaccaagaacaggcagaa
gaaagaaaacatgaatataaccccccccccccagactgcacgttaatcttgtagttgtgcgagcactgcaggatggggcaataactggtgtaggaagggt
aattgcctctaggatcacttgcattcggcaccggatagagtgttttagaaagcaaaaactgatataaaaagtttcagcactcaggacccaacacataatg
ggagatgttactacaaaccatatgagtagactgcaaggaggtcttggtgcatagttagactgctcgctaattcctttatagaccagcagccaaagcaatt
gccccctggcacagcagagatgggaaatactgagtgaacctgcctcacagcctatttcctcctgtaccanggagggatgtgagagaaaaaggacaaggag
tgagaggtttctccctgtcgctgtgcgtggagtacaaacgtcgcagactgagaatacgtggggcgcacaggcaaatgttccgtgagttcctgtgacgtca
aatcaggcgttacttgggctcttccactgtccctttgctgaagtcagccatctgggggtatttaactttcctctggtccagttcgttctgaacccagaga
gagcaccttgaacagttttgccagttttagggggtggactcccgggttctcataaatccaagacgggcttggttggacttcccttccataacctn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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