XB-CLONE-4352642
Clone Name: IMAGE:7027479
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Gene:
tnrc18
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784120 [+]
taattaatcccccccccccccccgttggtataaaatgtattttcttcgaattttgtttagacggcatcggaattggaatatggcaatgggacctggatgt
taaaggaacgatctacaatttataccttgtttaaaggcaagccttcctggatgtttcagtttaataaaccacggactctggatattacacttcccgaatc
acaaagaaatgacactagaatgagttgcagagcaacttagccagccgaaagggttatgcatatcctttgaaccatggcatggataaagagagtggcgtga
gaatggatggccgagagtttgtacacatacctccaccacttctacctgggcttcctttggagggagtccgaggtggagttgttggtgctactgggcgtct
tgtatcatctactggacatctttctccactgccaacaacaaagtacctaccaacagctctcactttacactcccacccaggttttccacacttagccaac
aatttttatccttcatacattcccataaatcaacttgatcctgctggcattcgaagccctctactgtctcagattggaaaccccagtatttttgagtcgc
aaaaagatggctttttaccttatctagccctgttaacccaaccatccctaaacttctcaaaattccatatcaagggacttttagcaaatccaggccccaa
aactcttcacccttaatggaaaagggaacccaccgggtcaactcgcaataaatgcctcttaagaaaaccggtggaaggaaaa
3' EST: CF784119 [+]tttttttttttttttacttcttttccttttacatctcgggcctgtgaagcaatttgtacaggcccattcctttcatcacgtgtgtcaactgaaggcacaa
aagtgggtgcagtgaccttgtgatcccttgcggactccttgactaaaggaaaggctgcacatgcagcagaacgggtgggatgtccagcaaatgtggaggg
tgtctgagagggctgtatagaaggacagctacacaatatagggatatttctatccgaggtccctaaactgtgacaagtcttagcttgatctttatatgtg
tgtcctgaaggcctcatattttcatcatgtctgctggtgtctttggcacacttatcctgctcatagcctgccaccttagtctgtggctcgccccctgtgt
tacagatatttgacaaagatgtttgaggtggatttttagcctttgtttcaacattacttgattcatgatttagcacaagctcggtcatatatggagacac
atattcccctgtcttagatatcctttgttgcccaccttttttcctttcactttcaagtctaccttccaaagcatagtcaagtacaactccgagcctgtgg
atcctctgttaatcttccttctttccttgatgaagttcaccataacctcatttctgcaaactcgctcttttggtttccttcaccgaattcctttaaaacc
atttatgccaagatgacccagtttgtggtcccttttccattaaggggtgaaaagattcttttgcccctgaatttgtctaaaaatcccctggaaaaatgga
aagtttttgaaaaaaaggtaaattgaagtggcctggggttaaaacaatgggcttataaaataaaattaataaaacccccttta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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