XB-CLONE-4352639
Clone Name: IMAGE:7027478
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Gene:
aldoc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784121
[+]
taattaatcccccccccccccgaccagatccagtgcgccgctctactcttagcgcctagcatcacccgaattcacgaaaatgactcaccagtacccagct
ctctctgctgagcagaagaaggaattggcagacatcgctcaaaagattgtggcacctgggaagggcatcttggctgctgatgaatccgtggggagcatgg
ccaaacgactaagtggaattggtgtggataatatcgaggagaacaggagactgtaccgtcagttgctcttcaaggggtgacgagcgtatgcaaggaatgt
tattgggtgggggtcatcttccttccatggaaaatatggtaccaaacaggggtgaactggcgggcaccaaatttttgttccagaaggaaaaaaagggaaa
aaaaggaaatttggttgtttgggggaattaaaaggttgaaaaaaaaggggggggggccccccccccttggccggggccccccaaaggggggagaaaaaac
ttaactaacttccagggggttttggggaaaaggggccttgggttcccaaaaaaccccctggggtgccccccggttttctccaaaaaaaagggatttgggg
cagccccaaaaaattttttgggcccccaaaaaaatgggggggggggttctccctgggggggcgcccaagaaaaaaaaaaattcctgggggggggaaaaaa
ataacagttttcttccccctttattgggtgggcccccccttgtgggggcacgaaatttttcctgggggggaaaaaaata
3' EST: CF784122
[+]ttttttttttttttcaatatataatttattttcatactatttggtacatcagtttcagacagagacagacaacacactagtttcacaagatacacagcca
ttcattagttgcccttcccaatcgctctcctttcagttctaatctaattttacttgccaaagacctaacaacatgggaaggacctgcttttagtggaata
ggaagcgcaacacttcttacactatctacctatagaagtaccgcaaagtgcaagacagaacagtgtcatggattgaatgaacagtagatatttgggtgca
gcttgtttttgtgcacgagaataataaaactggatatataccaccgtagaattatctccaactgcaccatatgttaatatttcctgtccaacaatggaca
cactattgacctatttagaaggattaccaacaatctgcactcctcttattatggctgtaaagcaaaatctttcacaaaaataaatgttttgttaggaaaa
tatactacaaccaggtacattatgctgcaaaggtgggtgaagcctgatccattcactcacaacctcacctccattacttcaagatcacattagcaacata
aggttagggggaataagctgggtaggagatggctgtgactattccaggctttgggctgcgggggatgtccaagtggaaggctgcttgcaggatgaggaca
acggttctggaggggagtgctcaggggactgcaaaagctccttatgcatgggtttgccacataaagggactgtccaggctgccccacatccatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: