XB-CLONE-4352636
Clone Name: IMAGE:7027477
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784124 [+]
taattaatcccccccccccccggcatccttgctctctcctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctata
ggtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggagg
aggctttggcagcggtgccgggtccagcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtggaaat
gaaaagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaagatcc
gcacttggcacgagaagcaaacaggcaccggccttggagctgggtctaaagactacagcaaatattttgagatcatcagtgagctgaggggcaagatcca
tggtgcaactgttgataacgctacagtgaccctgcaaatcgacaacgccagactggctgctgacgacttcagacttaagtttgagaatgagctggctctt
cgccagagcgtggaggctgatattgctggcctgcctaaagtcttggaagaactgaccctggctagaggtgacctggaaaagcagatttgaaagcttgact
gaagagctggcctacctgaagaagaaccatgaagaggagatgatgcctgccaaaaccagaacccccggcaaattgaatggttgaaatggaacccgcctcc
cggcgtgggac
3' EST: CF784123 [+]tttttttttttttttcagagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcaggtcttctctcttgtttcagg
ggaaatttctgttcatttgttcctttatctttcccgactgcccttgcatttatgacactgttgaagaaacgaccttcccatctaccacctcttcgacaat
ggtttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgtggtcatctgtcccagttcc
ccatccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgttccatgtcggacc
tgatctgcagaagctgttcctctaggctgctgatagtgttctgaatctgcagaagttgcgctgaatagaagccttgtaattcattgaggttgccttcaac
tgattgtttcattgccagctgggattgcagctcaatttccaagctctgaagtgaccgtttcagctcagtgatttcactcttgctggcctgcacttgttca
accccaactgaaatctctttcttcagctcaccgctcttctgggtgaaccacaactctgcatccctacggttcttctcagccagtatttcatagtcagctc
tcatgttattcaaaatgttggtcagatccacgcctggaagcgcgttcatctcaacactcacttttgccggcgctctgggctcttggcatgcatcatctcc
tccctcatgggttcttcttcaagga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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