XB-CLONE-4352616
Clone Name: IMAGE:7027470
|
Gene:
tuba4b
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784136
[+]
taattaatcccccccccccccccgggcatttctcacagagacccgtcgcacaggctccggtgagatttccccctgacttttctctaccgaacgaccgacc
ttctcccaagacatcaatcatgagggaatgcatctccgtccacgtggggcaggcaggagtccagatgggcaatgcatgctgggagctgtactgcctggaa
catgggatccaaccggacggccagatgcccagcgacaaaaccattggtggaggggacgattcttttaccacctacttcagtgagaccggagccgggaagc
acgtcccccgcgctgtgtttgtggacctggagcccactgtgattgatgaggtgcgggcaggcacataccggcagctcttccatccagagcaactcatcac
aggcaaggaagatgccgccaataactacgcccgggggcattacaccatcggcaaggagatcattgacccagttctggacagaatccgcaagctggcggat
cagtgcacgggactgcagggctttttagtcttccacagcttcggggggggcaaccggctctggattcacctccctgctgatggaaagtctctctggttga
ctatggcaagaaatcccaagctggaattctccatctatccagctccctcagttttcaaccgcggtggggggaaccctacaaatccattcctcaccaaccc
caaaaaaactggaagcactccaactggggccttctggggggaataataaagccctttatgaacatccg
3' EST: CF784137
[+]tttttttttttttttctgttctggagtttacttttttttcatttttgcaatttttttcactaaaataacctcagctgctaaaaataagatattcacatta
aaaataaaatccgagaggaagctggcaggatatacttctctagcattgggcttttacaggttgggaggggcagtgttaaactaggccaactggcaccttc
caatctttggaataataagatgatggataaagggcaattaatactcctctccctcatcttcatcttcataggagtcaatgccgacctcttcgtaatcctt
ctccagggcggccatatcctcgcgggcctcagagaactccccttcctccattccctcccctacataccagtgcacaaaggcacgcttggcatacataagg
tcaaacttgtgatccaggcgagcccaggcctcatcaatggcggtggtgttgctcaacatgcacccagctcgctgtaccttggccaaatccccacccggaa
cccctgttgggggctggtagttgatacccacctttgaaaacagttgggcaccagtccacaaactggaaggtaagttttggtccttgaaaccgccaataca
gcccttgcacatcttttgggcaccaaaaacaccgcgggtaaagcatggaaaaaagcccagtaattaaccaatgaaaggggggttcccattttcccccccc
catagatgggttgggcctcaatacacaacgcattagtgtgattcctccgaatacccgcaaaacctgcatccatggggaagggtctattctccctggctga
gatagaacatggggcgcattat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: