XB-CLONE-4352613
Clone Name: IMAGE:7027469
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784139
[+]
taattaatccccccccccacccccccggactagtctttgtgagtactccatctgcaggagggctcgcacatagaaaaaacaaatatatatatatataaat
aagtgcaagcaagccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcac
acaagccctcagtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcg
gctgtaagtgttacgaaaccagccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaag
ttccagcaaagggtaaaaagggagcaaagggcaaacagacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggagaaacaaa
aagtgacgaggcccctgcttctgatggaggcgacaaggaatcaaagtctgaataaccttcatcttgtaccgagtcttttatcagtggttcctgtacctcc
cttgtacaattcagaggaatatttttatcaactattttgtaaatgcaagttttttagtagtttaattctagaaaacacatttttaaaataggagggaatc
cccaccatttttttgttgtaaaaaaaatttttttttaaacacttatgaatttggtacattgcccctgtatgggttta
3' EST: CF784138
[+]ttttttttttttttaaagttcagagagaagttgtttcgtagaaaatctagcacccctcgtaccagcatctttgatgcaaataattcaacccatttctcgg
ccaagaaggagcgaacacgcataggtgccaccttggcaggaaagagatgcaaacacatgaaccgccgtgtcctttttgtttttctccctacttcacatca
cactcactacacactcaaagaagtttccccagaagagattcacagcacttttacaaagttactgaaaataaaataccactacccagaaaaaaaaaaaata
aaatggtaacatcacagatagtacacaattctttagagggaaatccgtcaccttgggagatttattagtttaaaagtgtaaattgtacgcaggacaacat
gagtacaaagaacagttaaaacgttaagacattttaatagccgtgatctttgcgccagttgtaccagtatcgggcactgcctgttcgaccggcagcctgc
atagagtactagccgttttgtaacaaaataaaaaaaaacattttagactgctcaaggcttgcactccccacagtaactggtttgtggtgtagtattaact
ggtatagtattcatagcctttacaaaccatacaggggcaatgtaccaaatcataagtgtttaaaaaaaattttttttacaacaaaaaaatgatggggatt
ccctcctattttaaaaatgtgttttctagaaataaactactaaaaaacttgcatttacaaaatagttgataaaaatattccctctgaanttgtacaaagg
gagggtacaggaaaccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: