XB-CLONE-4352606
Clone Name: IMAGE:7027466
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Gene:
sec11c
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077666 [+]
CCCCAGAAGGGAGGGCTGGATGTTGGCAGCGGGCAGGCACCTCCTATTCCCCTGCCAGTGACTGAGCTGCCGCCCAGCCCCATGCCCCCGGCTGCCCAGC
GCCTGGCATAGACACAGCCACCATGGACCTGTTCGGGGACCTTCGGAGGATGAACAAGAGGCAGCTCTACTACCAGGTTCTGAATTTTGCCATGATAGTA
TCTTCAGCTCTAATGATATGGAAAGGCCTCATAGTGGTGACCGGAAGTGAGAGTCCAATAGTCGTCGTGCTGAGTGGCAGCATGGAGCCGGCTTTCCATC
GGGGGGATCTGCTGTTCTTGACGAACTTCCAGGAAGACCCCATTCGCGCTGGTGAAATTGTAGTGTTTAAAGTGGAGGGCAGAGACATCCCGATCGTTCA
CAGGGTCATCAAAGTGCATGAAAAGGAGAATGGAGATATCAAATTCCTCACTAAAGGAGATAACAATGAAGTCGATGACCGAGGATTATATAAGGAGGGG
CAAAATTGGCTGGAAAAGAAGGATGTTGTGGGGAGAGCGCGGGGGTTTTTGCCATATGTTGGTATGGTCACTATTGTCATGAATGATTACCCAAAATTTA
AGTATGCGGTGTTGGCCATTATGGGCGCCTTTGTACTGTTAAAGCGTGAATCCTAACACTGGAAAAAGGCATAAGAATAATAAAGGCATAATATATGACT
AAAAAAAAAAAAAAAA
5' EST: CF784143 [+]taattaatcccccccccccccccccagaagggagggctggatgttggcagcgggcaggcacctcctattcccctgccagtgactgagctgccgcccagcc
ccatgcccccggctgcccagcgcctggcatagacacagccaccatggacctgttcggggaccttcggaggatgaacaagaggcagctctactaccaggtt
ctgaattttgccatgatagtatcttcagctctaatgatatggaaaggcctcatagtggtgaccggaagtgagagtccaatagtcgtcgtgctgagtggca
tcatggagccggctttccatcggggggatctgctgttcttgacgaacttccaggaagaccccattcgtgctggtgaaattgtagtgtttaaagtggaggg
caaagacatcccgatcgttcccatggtcatcaaagtgcttgaaacggagaacggaaatattacattcccctctacaggagatacacatgacattcgatga
ccgagggattatatacaggacgggtcacaaattgggcttgcaacattaacgatgtctgttcaggaaataaccgcgggggcgttttttgtccaccctggtt
tgggtttgggttaagctaattgggtcacggaaaccggatttacctcataaaacatctaacgttatttccaagaagatttgccgcccatttattggcggtc
ccctctatttgacgccctgttttcaccccccctcgaatttccttagaacagcgggggaaattattctcactcaccagtagttaatttttatttcgcccct
ccttttttctcccggcccccgttctgcacaacccctagtatccccacgtgaaatcccctcctcacaatctctcttttagatgctgtgagcttcgtgtaca
attcacagcgcggatagtacaacaaactcttggcaggttgatatgttcccaccccacccacactctttaaaatttttttatcgcagaacatcacgctttt
tt
3' EST: CF784142 [+]tttttttttttttttagtcatatattatgcctttattattcttatgcctttttccagtgttaggattcacgctttaacagtacaaaggcgcccataatgg
ccaacaccgcatacttaaattttgggtaatcattcatgacaatagtgaccataccaacatatggcaaaaacccccgcgctctccccacaacatccttctt
ttccagccaattttgcccctccttatataatcctcggtcatcgacttcattgttatctcctttagtgaggaatttgatatctccattctccttttcatgc
actttgatgaccctgtgaacgatcgggatgtctctgccctccactttaaacactacaatttcaccagcgcgaatggggtcttcctggaagttcgtcaaga
acagcagatccccccgatggaaagccggctccatgctgccactcagcacgacgactattggactctcacttccggtcaccactatgaggcctttccatat
cattagagctgaagatactatcatggcaaaattcagaacctggtagtagagctgcctcttgttcatcctccgaaggtccccgaacaggtccatggtggct
gtgtctatgcccggcgctgggcacccgggggcatggggctgggcggcagctcagtcactggcaggggaataagaagtgcctgcccgctgccaaaatccag
ccctcccttctggggggggggggggggggggatttaattatttctnatggcgaaaagaaaaaaggaatcttggttttccggccccggaataaaacccaat
ggtcctttttgaccctgggaaaatggaaacctcggggaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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