XB-CLONE-4352603
Clone Name: IMAGE:7027465
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Gene:
eif6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077665 [+]
GGAGAGGAGCCTGTAACTTCGGGATTGTGTGTAACGCACGAGGTTCGGGGCCGGGAGTTGCCGGGAGTTGTCTGGCTTGGCTGTTTGCGGGATCGTGCAT
CAGTGATTTCTAAGAAGCAGACATGGCAGTCCGTGCATCTTTTGAGAACAACAATGAAATTGGCTGTTTTGCCAAGCTTACCAACACCTACTGCCTGGTG
GCCATTGGGGGATCGGAGAACTTCTACAGTGTGTTTGAAGGAGAACTTTCCGAGACAATACCTGTTGTACATGCATCTATCGCTGGCTGTAGAATAATCG
GGAGGATGTGCGTTGGCAACAGACACGGCCTCATGGTTCCAAACAACACAACAGATCAAGAACTTCAGCACATCAGAAACAGCTTACCAGACTCGGTGCG
AATTCAGAGAGTAGAGGAGCGCCTCTCTGCCTTGGGCAATGTTATTGCATGTAATGACTACGTGGCTCTTGTGCATCCTGATCTTGACAGGGAGACAGAA
GAGATCTTGGCAGACGTCCTTAAAGTTGAGGTTTTTAGACAGACAATAGCCGATCAAGTACTTGTAGGGAGTTACTGTGCCTTCAGTAACCAAGGAGGCC
TGCTGCATCCCAAAACATCTATTGAGGATCAAGATGAACTGTCTTCTCTGCTACAGGTCCCTCTTGTGACGGGAACTGTAAACCGCGGCAGTGAAGTGAT
CGCTGCAGGAATGGTTGTGAATGACTGGTCAGCGTTTTGCGGCTTGGATACCACAAGTACAGAATTGTCCGTTATAGAAAGTGTATTTAAACTGAGCGAC
GCACATCCTAGTACTATTGCCACCAGCATGAGAGACTCCCTCATTGACAGTTTAACATAAAAAAATGGCAAGCATCCCTGATCGTTCCTTTGCTCAAACA
AACAACTCTGTCGCTTACCAGCTTTTTCACCAAACTGGACTTCCCAAGAGCGCTGTCGCCCAAATATGTCTTTGTTATTCTGTCATCGCATTAAAACTGT
ATCTGAAATAAAAATGAAATGGCTTTTTTTAACTGCAATTTGTGACGGGGAAAAAGAGTGTCGTGTAAGCATCTGCTTGCTATATTGCACTTAAATATAT
TCTAGGTTATTATGAATAATAAACAGTTTTATTACTCGAAAAAAAAAAAAAAAA
5' EST: CF784145 [+]taattaatcccccccccccccggagaggagcctgtaacttcgggattgtgtgtaacgcacgaggttcggggccgggagttgccgggagttgtctggcttg
gctgtttgcgggatcgtgcatcagtgatttctaagaagcagacatggcagtccgtgcatcttttgagaacaacaatgaaattggctgttttgccaagctt
accaacacctactgcctggtggccattgggggatcggagaacttctacagtgtgtttgaaggagaactttccgagacaatacctgttgtacatgcatcta
tcgctggctgtagaataatcgggaggatgtgcgttggcaacagacacggcctcatggttccaaacaacacaacagatcaagaacttcagcacatcagaaa
cagcttaccagactcggtgcgaattcatacagtagaggagcgcctctctgccttgggcaatgttattgcatgtaatgactacttggctcttgtgcctcct
gatcttgacagggagacacaagatatctttggcagacgtccttaaagtttacgtttttacaccgataacacccttatctagtaatttgtaaggaagttac
ttgtgcctttttagtaacccaaggatgggtctgcctgcctcccaagaacatcttattttttggcttttcgcccgcaccttgttctttccctctgggtata
agagttcccctccttggttaaccgtggtccaccgactataatcccccgcggctgtttgacaacctcgtttcccccctttaatgcgcctctcgtgtctgtt
tttgaaattgtgaactgtgttgctaattccccttttactgcacgcaacattttcgggtttttttttcccaccccccccctcttccgcgttatatttttcg
tcaaaccccccggtaaaagac
3' EST: CF784144 [+]tttttttttttttttcgagtaataaaactgtttattattcataataacctagaatatatttaagtgcaatatagcaagcagatgcttacacgacactctt
tttccccgtcacaaattgcagttaaaaaaagccatttcatttttatttcagatacagttttaatgcgatgacagaataacaaagacatatttgggcgaca
gcgctcttgggaagtccagtttggtgaaaaagctggtaagcgacagagttgtttgtttgagcaaaggaacgatcagggatgcttgccatttttttatgtt
aaactgtcaatgagggagtctctcatgctgatggcaataatactaggatgtgcgtcgctcactttaaatacactttctataacggacaattctgtacttg
tggtatccaagccccaaaacgctgaccattcattcacaaccattccgggcgcgcccatttcccgggccggtgttttaacggtttcttgtccccacgaggg
aaccctgtaatcccaagaaagacaattttcattccttggatcccttcaattaaaatggtttttttgggggatgtgcaggcacaggggcccactcatttgg
ggtgcaacattggaacagggccacaaagggaaaaacctctcctcttttaaccaacggacaagtcgcttcaggatatttgggaacgtttactggcggggat
acttagtgccggccgcgcggaaaataaatcacctttaaaaatatacacactttcacgcgtaacaaagggggggaaaacccggcagcatgccaccggggga
gccctctcacaaaaaagagaaaaaaggacagtccgccgtgtcgcccaatgtgatcccccccatgacccggaaatggtgtaatttccccaacgaaggtaat
ttttttaaggaggtgtagtccggccctcaatcttatgagcgcgaacgtcgttcccgaaccagatattgaaaaattctacccacgttcataaacgggagtc
gctgtaaaaaatacaccaccatatctgtagacacaccccaaaaaggggttctatgaaaagagcggagaaggacgatgcactgtccccaaaaaaaaaaact
nacgcaagaaaaaactccggagaaaatcgcatgttattttacacacgcctcacgagcggatgtgtttttatctttcataagaggcaacttaaccaacccc
tctcctttacacccccccccggacgctttttattatataaaaaaaaccgggcggctg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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