XB-CLONE-4352600
Clone Name: IMAGE:7027462
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Gene:
serpina1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784147 [+]
taattaatcccccccccccccgctgatctggggtagacctctggatacagccagattaaagataggaagaacaatgagggcctttttgattgttagccta
gctctgctttgtgcaggggtcctggctgaccatgatggtcagaccaaacatggtaaggaccatgatcacaatggccatgaccatggagatcatgaccatg
accatcatcaccatggtaaaggaaaacatcacgatcacaagcaccatcatcatgctggtgaagacatggcttgccacaaaatagcaccatccaattccca
atttgctttcaaactcttcagacaagttgtagcagatcatccatcggaaaacattttcttctctccagtgagtatctccactgccttggctatgctctct
ttaggtgccagagctgacactttaaaccagatcatagaaggactgaactttaacaacactaaaataacagaggaggagatacataacggcttccagcatc
tccttcacatgctgaatgacccagacagagaattgcagctgaacagtggaaatgccctcttcatagacaataatgtgaagctcatccaacaattcataga
tgatgttaaaaagtactatgaatcagaagctttttctactgactttcataatgcagaggaagccaaaaaacagattaacatttatgtggagaagcaaact
cacggccaaaattgtttgatttggctcaccaagtgtcgaataaaaaatgccggttttaaaaatcttaattaaacctaccttttttctttcagaagggaaa
aatggggaacaaaccccttttttgaaaaaaaaggtttt
3' EST: CF784146 [+]tttttttttttttttcgacgacatatgttattttattacacaaacaatgacatgtaaactaatttttggggtttactacttttgcaacaaagaggatgct
ttttggaataggatcataaataattacaacgaaggcccgattaaacaagatgtgtggtgggatcatcattggcataatctcaaaagcagtagctgctgct
gcctccgttcctgtctcatcaacgctcaaaccagccttatgaactgccttagacaccttgagtggggttccttccacaatcccagagagatcagcgtttt
gtgaaaaaacatcagtcactccaaatttcttaagctcctcgataagatcaagtgttgccataattgaaaatttggggatagttaactctatagattgata
tctaaattgttttttccaggacattattgttgacttttctagagcttcttccacttgtctcagctttccttcatctggcaaaataaagagtgctgttgca
ttccctttgtatgggatttgtaccacagtgcagccaagcttttcatcaaatgccacattgtacatgccatttctgcgcatcattgggactgtcacatttg
tgttctcgtccacatggaagattccatcctgtgtaaacttttcttcaaaaggcttttcccattttcctctgaagaaaatgtagttaataagatataaaac
agcatttttatcgacactgctgagcaaatcaacaatttttgccgtgagtttgcttctcccacatactgttaatctgtta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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