XB-CLONE-4352595
Clone Name: IMAGE:7027460
|
Gene:
h3c13
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784150
[+]
taattaatcccccccccccccacgttagtttgtctcctcgtttgaccgtttgggttacgctcccgggagtgatcgaataacaagaagcaatggctcgtac
aaagcaaactgcccgtaaatccactggtggaaaggctccaaggaagcagctggctacaaaggctgccaggaaaagtgctccttccacaggtggtgtgaag
aagccacacttatacaggcctggcactgttgccttgagagaaatcagaagatatcagaagtctactgagctgttgatccgaaaactaccattccagcgac
ttgtaagggagattgcccaagacttcaagactgatttgaggttccagagtgcagctattggtgctcttcaggaagcaagtgaggcttacctggttggcct
ctttgaagataccaacctgtgtgccattcatgccaagagagtaacaattatgcccaaggacatccagttagcaagaagaatccgtggcgagcgtgcttaa
acattttgctaggaacttttttttgaacttagtttttttttttacaattgtacatacaagtgtgatgtcttttattttataaagggtttgttaactgtag
agtagttgacagtaagatggtagtaaacattttatatgacattcccttaatcctcaggtttttcagaaatttgtatctgcagctagtcttttgtgaccct
ctcctttaaaacatagtgcatgcccatacttcattctttacataatttagtatctttctgtgtaactccattgtaaataaacttttccactaccctcaaa
caaaaaaaaaaaaa
3' EST: CF784149
[+]tttttttttttttttgtttgaggtagtggaaagtttatttacaatggagttacacagaaagatactaaattatgtaaagaatgaagtatgggcatgcact
atgttttaaaggagagggtcacaaaagactagctgcagatacaaatttctgaaaaacctgaggattaagggaatgtcatataaaatgtttactaccatct
tactgtcaactactctacagttaacaaaccctttataaaataaaagacatcacacttgtatgtacaattgtaaaaaaaaaaactaagttcaaaaaaaagt
tcctagcaaaatgtttaagcacgctcgccacggattcttcttgctaactggatgtccttgggcataattgttactctcttggcatgaatggcacacaggt
tggtatcttcaaagaggccaaccaggtaagcctcacttgcttcctgaagagcaccaatagctgcactctggaacctcaaatcagtcttgaagtcttgggc
aatctcccttacaagtcgctggaatggtagttttcggatcaacagctcagtagacttctgatatcttctgatttctctcaaggcaacagtgccaggcctg
tatctgtgtggcttcttcacaccacctgtggaaggagcacttttcctggcagcctttgtagccagctgcttccttggagcctttccaccagtggatttac
gggcagtttgctttgtacgagccattgcttcttgttattcgatcactcccgggagcgtaacccaaacggtcaaacgagagaaactactggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: