XB-CLONE-4352590
Clone Name: IMAGE:7027457
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Gene:
mmp25
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784153
[+]
tcctcctcatcttcctcctcctcatcctccaacaaagccagcagcaaaaaagcagcgtctcccgttactagaagatcacgcagcccacggaaacctatcg
actctctgcgtgactcccggtctctaagttactccccagaagaacgtaggcgtcaaccatctcccccacccccgcgaaggagagaaagggattctgggag
gaatcaggagtcgcctgaacggaagaggcgaaaggactcgcccaactccagatactctcacaaaagaacttcgaggtctccgtgatccgtcgccatcgag
gaattttcataactgtttcctccatctatctgtaatgactatgggacttggttcgttcggtgacccttttttgcctgcaagggtgcagactatttactta
catattgcttttttttggtgggggagggagcctgaggcagctgaaattgagactgataacagggggcctgtgactcccctgaacacagtgtgtctgtctg
cactacactgggaatggctccccctgcccataccgccctgaggatcttacgcccagaagatgaaacgtgaacttcattagggtggtggtactcctccctc
agagggtgattgccccccagccttccttttggggaggcgccaatctagagaactgggtcaaatcccccccctcgtttttccaccccagggtttacgccct
tttatccgacaatctgccctaaccccctggctttctacctacattcttactccgacacctgggcttattacgcttattttcctcctaccctactgtgtgg
aatttttcgtacgaaactgtggaaaacaggtttattaggtccccgcgggcgctccaaaagttcttgtgtttttcccgctccgcccccgcgcgttaattac
ggga
3' EST: CF784152
[+]ttttttttttttttcgtttgttttttctctggttttgcccattcttttattgacagaaaggatttctctcgctgtacagattacaaaaaaaaaaaaaatt
aaacacaaacataagtctcacttttttaaattcacttattacgaaattgcttatgcagaacgaaagatgggaagagcagggctgggcagtgtcagactga
cgtaacctagatggagggagggggggggtatacgcagtctctagatggcacctcccagaagagggctgggcggagtcacctgtgagggaggataccacca
ccctaatgaagttcacatttcatcttctgtgtttaagatcctcagggcggtatgggcagggggagccattcccagtgtagtgcaggcagacacactgtgt
tcaggggagtcacaggccccctgttatcagtctcaatttcagctgcctcaggctccctcccccaccaaaaaaaagcaatatgtaagtaaatagtctgcac
ccttgcaggcaaaaaagggtcaccgaacgaaccaagtcccatagtcattacagatagatggaggaaacagttatgaaaattcctcgatggcgacggatca
cggagacctcgaagttcttttgtgagagtatctggagttgggcgagtcctttcgcctcttccgttcaggcgactcctgattcctcccagaatccctttct
ctccttcncgggggtggggggggaaatggtttgacnccntacgttnncttctgggggagtaancttannagaccggggagtcnacgcagagnnagtcgat
aggggtttcccgtggggggctgcgntgatctttcttagtaaacggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: