XB-CLONE-4352587
Clone Name: IMAGE:7027456
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Gene:
olfm4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784155 [+]
taattaatcccccccccccccctagagaggacaggattagagagcaaaatgctgcagcttctaatcattgctctggggatttgccaggctgcctcactgg
caatatcaactcctgggtctcttgatgagcatggagtctgtcagtgttccttgactgtcccagatatgacctttcctgctgatcgaatggagattctgga
aagagaaaacatgaaactgagtgccagtgtccaggaacagatcactaagatagaagaatacaaggccacactaacaggatgcctgcagaagctgaccaat
ctgaccaagaaggttgaatttatggaaatgggaggcatctcctacacagagcttgactttgagctgctgaagctagagattaaggaaatgcaaactttag
tagaacaactcagattttctatcaatggttccagtgctttagtggagagtctctttgaggagatacataacatctccattatggtcaaccatctggagtc
atatgataaaaacaatgtgctagctgtgcgtcgtgagatagctgcccttcggaagcgcctggaagattgtgagaagaaccgtgttactccaacacaacct
tctatcagttatggaacttgcaaacatggaggattggcaaacatcagtaaaaccttttgtggtgcacctgaactggctgggttttttccttacaagtttt
ggaggattggggtaaaggacacattttccaggaacccaatccaagataaatcatttgggtaacctccttctgccaacctgaagggaacggataatggaac
attttgtgaaaatttttaacactttcataatgaataaaccctctttggct
3' EST: CF784154 [+]tttttttttttttttataggcatttttttattatttaatttcttcataatgcaaaataaaaaaaaaaagcatttaataaaacactatggaattacagtgc
tgataaatgtggtaaagattaatagttctacaaatatttagagaaaacaatagaatgcagagtttcttatgtaaaagaaactgctgtaaatggagtgcaa
aaaaagttaagttaagcctcaattttgacccaacttgccatttgtaaacctgaatataaagtctgatttaagcctgattcaacaatggtttaaagacaag
atcataggtgattaagtaagcatcattgaacataaagagcttttgatcattagggttatatgcaagactctgaacattttccatcatcttgtccagaatt
atgctcagctgtccttccttattggtatttgtgtcatacatgtagaatatctcttccttccgagtattaagtgttctagtggcataaagaacaccacaaa
ccatgaaggcatttgttgctccaagcttatactgagaagtggaccaagtcttttccactgttaaggaggtggaattgagtttgctaatcacaatgtttcc
agagttttgttcagtggaataaattgcccagagtccattttcatcactggctagatcaatatcttgccagctggaggatgcatagngagaaacggttatt
gaaagaaagcgtctggtagtggcttacgttctacaacattagtgtccacactgtatttgcagatgtctctgctgttgtaaacgtt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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