XB-CLONE-4352578
Clone Name: IMAGE:7027453
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784161
[+]
taattaatcccccccccccccgagtagccaaatccaaccgttaaagcatcagcagtttaatgagagtatacgttctcataataaagaaatttgttctgta
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaggggtccccttt
ttttaaaggggccccccccccgggggaacttggccggggcccaatttgtttattggaccttaaaagggttaaaaaaaaagcaaaaccctcccaaatttca
aaaaaaaagcatttttttcccgggattcaaattggggtttggccaaacccaccaagggatcttaacagggcgggaaggggaataaattcggcgcaacccc
agggcctgaaaaaacctctaaaaaaaaaaattggttaggtaccttttgaggggaaaaaaaccacctggggaaagggggtcaattaggggggggaaaatcc
ccaggctccccaccggggaaaaatttgcaaagattgcttctcaattaatcaccaaccagggggggaaattccccaggcccccccccggggaaaaatatgc
aaaacatgcctcccaatttgtccgaaacctaagtccccccccaaaatccgccaatcccggcctaaaaatcccccattttcccccaatttcccccccccag
gggggaaaaaattttttttttttttatgcaaaaaggccaaagcccccccctcggcccccttaaaacttttccccaaaaaaattgtgtggggggggggttt
ttttggggagaaaaaataaaagctccctaaagggttttttttgaaaaaaaa
3' EST: CF784160
[+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttacaaaaaaaat
ttttttttttagaaaaaggaaaccccccttaaaccggggaagctttaaaggggggaattggcccccccgggggggggggggataattaaccccgggggaa
aaaaaaaaaatttctttttttccggccccgaaaaaacccaagggccattggacccgggaagggacccccggggaaaaaaaaggaaaagggaattttttcc
taaggggcccccaaaaccgagggggaaaaaacaaaagggttttttttaattgggccccccttttaaaaaaacccagggggggggcccaaaaaccccaaag
gggggactttccccccccttgggcgggggaaaccccgtttcaaaggccccttccccgccccggaagggggaaccccccccggggcttccttggggggcaa
aaacccgggggaggggccccccggggaattgaagggttcaaaaaaaagccctgctttaaaaaacccccccccggaaacccccaccccccattttggtcaa
agggggcggaatttttaccaaaatttggaaaaccccctttaatttggggcccaaaccaaccccccttggccccaatggggggggaaaatggaaaaccccc
ggaagtaaaaaccgctatcccccccccttggttgttccgccccaaaccccccccccccggggaaaaacggagagaccaaaaaccctaaataggtcccgcc
cacataaaaaaaaaccccccaaaagggccctgttactgggggcaaaaaggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: