XB-CLONE-4352571
Clone Name: IMAGE:7027450
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Gene:
ston1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784165 [+]
taattaatccccccccccccccccgctcattgcaagctttacaagctcggagacggcgagcagctcatttaaaaccagtcgtccatatcacattactgag
agcaacaatgtctaagggaccagcagttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgccaat
gaccaaggaaacaggaccacacctagttatgtggcttttacagatactgagagattgattggagatgcagctaaaaatcaagttgccatgaaccccacaa
acacagtttttgatgccaagagactcattggccgcagatttgaagatgcagttgttcagtctgacatgaagcactggcctttcaatgttatcagtgacag
tggaagaccaaaggtccaggtagaatataaagcagagaccaaatctttctatccagaggaaatatcatctatggtcttagttaagatgaaggaaatagct
gaagcataccttggcaagactgtaacaaatgctgtaattactgtcccagcatattttaatgactcccagcgacaggctacaaaggatgctggtactatat
ctgggctgaatgtactgcgaatcattaatgagccaactgctgctgccattgcttatggacttgataagaaagttggagcagaaagaaacgtgctgatctt
tgatcttgggtgggggcacctttgaagtcttccttctaaccattgaaagatggcatcttttaagtaaagtcctacccgctggtggacactccattttggg
gtgggtggaagaacttttga
3' EST: CF784164 [+]ttttttttttttttgccaattcaattcattttattggtctggacccaatttgtttgtacagtacagatactatggttttcctgacttttggagttaattc
aagtattaatgccccaaaattgacatacagcagcatacatgagaaaatttgaacagctagtacgaaatttgtgtcctaggagttacaagacaaaaaaagt
ggaatgcagcttttatttagtcaacctcttcaatagtaggaccagaggatgctcctcctgatggggcaccaccagcacctgggaagccaccaggcatacc
acctggcatgcccccagcactttgatatagctttgtaataatagggttgcacaccttctccagctctttttgttgatgttcaaactcttctttttctgca
gtctgatttttgtccagccaagatataacttcattgcatttatccaggattttctgtttgtcttcatcactaatttttcctttaagtttctcatcttcaa
cagtagccttcatgttaaatgcgtaagactcaagggaattctttgaagataccttatccctttgcttctcatcttcagctttgtacttctcagcttcttg
aaccatacgctcaatatcttccttgctgagacggcctttgtcattggtaattgtgattttgttctcctttcctgtgctcttgtcaacagcagacacattt
aagataccattggcatcaatgtcaaatgttcacctcgatcttgaggaacacctccgggaagctgggagagatgcccgtcagctcaaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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