XB-CLONE-4352568
Clone Name: IMAGE:7027449
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Gene:
kcnk1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784167 [+]
aattaatccccccccccacccccccacatcagctctccgttcccagagagacgctatgttcccctcgcagagccgtgaattttccataacataatcagga
aatgatgctccaagccctggccaccaacccctgcctgcggctgatccaacggaaccagacatgctggtgctttgtcctcttgatgttgggctacctgctc
ttccttctgataggggcagccatattctcagcggtggagctgccccacgaacacgttctccgggaggagctgctggacttaaagcatcgctacctgcagg
agaatgagtgcctgagcgaggagaggctggagagcttcctcagccgagtactggaggccagcaattacggggtgtccatgctgaacaatgtctctgggaa
ccccaactgggacttcacctctgccctgttctttgttagcacggtgctctctactacagggtatgggcacacagtgccgctctctaacgcagggaagacc
ttctgtatcatttactctaacatcgggatccctctgaacctgctccggttcactgcgctggtgcagtggatcatggtgcacgtgaccccacgggcccttt
cctacttccaccctcgctgggggtacaaccaaaccaatggggccctcattcaacgcccggccatttggggttagcggccattttgggggctttttcccca
aaccgggccggaattttttttccgggttttcgaaaaagaatttggaacccttcccaaaatatcgggtttaaacgcccgggcttcctttttttttctgaac
gcgcccttctgggttcgtgggcaaataaaaatttttccccccaccccataatagtggcca
3' EST: CF784166 [+]taacgagcgccagtctgtcctactaatcactatttagtgggccatcgggcacagagtgttcgtccagcatttgttcctcctttaaggacgccgcttgctc
tgaaatggaggcgagggtcagctgatcgtgttctatgatgtttaattggtctccctctttcattttcttcctgtagaacatttttcggaatttcttcagc
ccctgcagctcacagaaagtctccagaaccacaagcatcacaatgaggcccaatatcaggtaacaagtgatgccgaacttgtacagctggcggtacctct
ggttttgcccctccgcgggcacatagtctcccaggccgatggtgctcaaagaaatgaagcagaagtaaaacgactctaggaaattccaatcatcttcgag
agccgagaaaattgcggccggtatgaggaaaaagcacaaaatggccacaaacccaatgaccagcgcgtgaacgacggccacagtctgcttgttgtacccc
cagcggaggtggaagtaggaaatgggcctgtgggtcacgtgcaccatgatccgctgcaccagcgcagtgaacaggagcagtgtcagagggatcccgatga
tagagtaaatgatacagaaggtcttccctgcgttagagagcggcactgtgtgcccataccctgtagtggagagcaccgtgctaacaaagaacagggcaga
ggtgaagtcccagttggggttcccagagacattgttcagcatggacaccccgtaattgctgggctccagtactcggctgaagaaagctctcagcctctcc
tcgctcaggcactcattctcctgcag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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