XB-CLONE-4352557
Clone Name: IMAGE:7027444
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Gene:
rpl10
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784174
[+]
taattaatccccccccccccgtctttctggtgttggcgaggtgcagccgccatcatgggccgccgacctgcccgctgttacagatactgcaagaacaagc
cgtaccccaaatcccgtttctgtagaggagtcccagatcccaaaatccgcatctttgacttggggcgcaagaaagcaaaggtggatgagttcccattgtg
tggtcacatggtatctgatgaatatgaacagctctcttcagaagctctggaagctgcccgtatctgtgccaacaagtacatggtgaagagctgcggcaag
gatggcttccacatccgggttcgcctgcatcctttccatgtcatcagaatcaacaagatgttatcatgtgccggtgctgacagacttcagaccggaatgc
gtggtgcttttggcaaaccccagggcacagtggcacgtgtgcacattggtcaagtgatcatgtctatccgcaccaagacccaaaataaggagcatgtgat
tgaagctttacgccgagccaagttcaagttccctgggcgtcagaagattcacatttctaagaaatggggttttaccaagttcaacgctgacgagtttgag
tctctgcttgcggagaagcggcagatccctgacggctgtggggtgaagtacatttccnaacaggggcccccctggacaaatggagagccattcatgctgt
ttagattctacttctagcagagccctctctcccaataaaaactatttgggatgtttgaaaaaaaaaaaaaaaaaaggatcccactagtttctagaagcgg
gccgccccaccgccggtgggaagactt
3' EST: CF784173
[+]tttttttttttttttcaacatccaataggttttattgggagagaggctctgctagaagtagaatctaaacagcatgaattgctctccatttgtccagggg
gcccctgttgggaatgtacttcaccccacagccgtcagggatctgccgcttctccgcaagcagagactcaaactcgtcagcgttgaacttggtaaaaccc
catttcttagaaatgtgaatcttctgacgcccagggaacttgaacttggctcggcgtaaagcttcaatcacatgctccttattttgggtcttggtgcgga
tagacatgatcacttgaccaatgtgcacacgtgccactgtgccctggggtttgccaaaagcaccacgcattccggtctgaagtctgtcagcaccggcaca
tgataacatcttgttgattctgatgacatggaaaggatgcaggcgaacccggatgtggaagccatccttgccgcagctcttcaccatgtacttgttggca
cagatacgggcagcttccagagcttctgaagagagctgttcatattcatcagataccatgtgaccacacaatgggaactcatccacctttgctttcttgc
gccccaagtcaaagatgcggattttgggatctgggactcctctacagaaacgggatttggggtacggcttgttcttgcagtatctgtaacagcgggcagg
tcggcggcccatgatggcggctgcacctcgccaacaccagaagacgggggggggggggggattaaattaactcgagtgcac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: