XB-CLONE-4352554
Clone Name: IMAGE:7027443
|
Gene:
prdm1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080145 [+]
GTCTAGATTTAAAGAGCCATTTGGAGTGTGTGTAAGCAGCAGTAGGTGAATCAGAGCAGACACAAGCTCAGATTGTGCACATGGACTAATACTATGGAAT
GCTTTTAGTGTTGACTGGATACCTGCTTTATGGAAGTACATCAAGGCTGCCACACAGCAAACAGCAATTACAGGTTCTGATACTCCCTGACAGGAGGAAA
CATACTAAACAATGAAAATGGATATGGAAGGAATTGATATGACGTTGTGGTCAGAGACTGAATTTGAAGAAAAGTGCACATATATTGTGAAGGATCACTC
CTCGGACAGCAGCTCAGAGGGCAGTAATGTAGCTCAGGCACAAGCATCTTTGCCCAGGAATCTGCTTTTCAAATATGCATCAGGATGCAAAGAGGTGATT
GGAGTAGTAAGCAAAGAGTACATACCAAAAGGAACACGGTTCGGACCACTTGTAGGGGAAATCTACACAAATGACACTGTTCCAAAGAATGCGAACCGAA
AATATTTTTGGCGGATCTATTCAAATGGTGAATTTCAGCACTTCATCGATGGTTACAATGAAGATAAGAGCAACTGGATGCGCTATGTCAACCCAGCACA
CTCTCTTCAAGAGCAAAACCTTGCCGCCTGCCAGAATGGCATGAACATCTATTTTTACACCATTAAGCCTATACCAGCCAACCAGGAACTTCTGGTGTGG
TATTGCCGGGACTTTGCTGACAGACTTCACTATCCTACTTCTGGAGAACTTGTAGTGAATCTCCAACAAAGCCTGACCATTAGAGAGGAGAAAAGGAAAG
AATTGACTCAGCAGAAAAACACTCCAAAAAAGGAGCACAGCGTGAAAGAAATCCTAAGGGATACAACAAGCCACTTGAAACACAAGGACAATCTACTGAG
CTCTATGTCTGCAATCACCCCAGAAAAAGAAAAAGTGGATGTGCATAAAAACTGTAGCCCTGAAAGGACTTTCTTCCCTAGGGTAGTTTACCCATTCCCC
TCTCACATTCATGAAGACTATTTAAAGGCATCTGTAGGCTATAACATGGATAGACAAAATTATCTGATGCATTCACCAATTCAGCCATCCACAACACCAA
GTCCTTCATCAAGAAGTAGTCCTGACCAAAGTTTTAAAAGTTCAAGTCCTCACAGTAGCCCTGGATCAGCAGTTTCACCTCATCATCCACTGCAAGATCA
CAAAGAATTCTACCCATTCATCAACAGACCATATAATACTGAAGGTTTGGGGTCATTTCCAACTTATGCACCCCCAACAAGTCACCTGCCTCCATTTGTA
TCCTCCTACAATTCAACGTATTCTAAATATTTATTACCACCATATGGCATTGGCTGCAATGGACTAAATTCATTGAACAATATTAATGCTATTAATAATT
TCAACCCCTTCTCAAGAATGTACCCAGTTTACAGCAGTATGCTAGCGGGAGGAAGCCTCCCTCACCATTTGCTAACCCATGCTGCCCTCCCTGGTTCCTT
ACCACATGAGGGAGGACGTAGATTGCTGCAACCTGAGCTGCCACGAGACTTCCTTATTCCTGCACCCAATAGTGCCTTCTCCATCACAGGTGCTGCTGCA
AGCATGAAAGACAAGCAGAGCAGTCCCACAAGTGGATCACCAACTGCTGGCACAGCTGCATCCTTGGAGCACATAATGCAACCTAAACCTACCTCAGCAG
TGATGTCCACAAGCAGTGAGGAAGCCATCAATCTCATTAAGAGCAAAAGGAATATGACTGGTTATAAGACTCTCCCATACCCACAAAAAAAAAAAAAAAA
5' EST: CF784176 [+]taattaatcccccccccccccgtctagatttaaagagccatttggagtgtgtgtaagcagcagtaggtgaatcagagcagacacaagctcagattgtgca
catggactaatactatggaatgcttttagtgttgactggatacctgctttatggaagtacatcaaggctgccacacagcaaacagcaattacaggttctg
atactccctgacaggaggaaacatactaaacaatgaaaatggatatggaaggaattgatatgacgttgtggtcagagactgaatttgaagaaaagtgcac
atatattgtgaaggatcactcctcggacagcagctcagagggcagtaatgtagctcaggcacaagcatctttgcccaggaatctgctttccaaatatgca
tcaggatgcaaagaggtgattggagtactaagcaaagagtacctaccacaaggaacacggttcggaccatttgtaggggaaatctacacaaatgacactg
ttccacagaatgcgaaccgaatatattttttggcggatctattctaagggggaatttcagcacttcctccagggttacaatgaaaataatagccccctgg
ttgcgcctatgtctaacctaccacattttttcttcaagaacttaaacccttggcctccttgccaaaaagggcgttaaaaatttccattttttacacccga
ttaaaacctaattacccgcgccaaacaggggaactccccctgggggccggtatttcgccccggaaactctggcctgacttgttatttcccccaatacttt
actttcttggggacccaaccttttctaattgtacatcctcccccaacaatac
3' EST: CF784175 [+]tttttttttttttttgtgggtatgggagagtcttataaccagtcatattccttttgctcttaatgagattgatggcttcctcactgcttgtggacatcac
tgctgaggtaggtttaggttgcattatgtgctccaaggatgcagctgtgccagcagttggtgatccacttgtgggactgctctgcttgtctttcatgctt
gcagcagcacctgtgatggagaaggcactattgggtgcaggaataaggaagtctcgtggcagctcaggttgcagcaatctacgtcctccctcatgtggta
aggaaccagggagggcagcatgggttagcaaatggtgagggaggcttcctcccgctagcatactgctgtaaactgggtacattcttgagaaggggttgaa
attattaatagcattaatattgttcaatgaatttagtccattgcagccaatgccatatggtggtaataaatatttagaatacgttgaattgtaggaggat
acaaatggaggcaggtgacttgttgggggtgcataagttggaaatgaccccaaaccttcagtattatatggtctgttgatgaatgggtagaattctttgt
gatcttgcagtggatgatgaggtgaaactgctgatccagggctactgtgaggacttgaactttttaaactttggtcaggactacttcttgatgaangact
tggtgttgtggatggctgaattggtgaatgcatcagaatattttgtctatccatggttatagcctacagatgccttttaaataatcttcatgaaatgtga
aaggggaaatggggtaactaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: