XB-CLONE-4352541
Clone Name: IMAGE:7027436
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784183
[+]
taattaatccccccccccccctcattcagccggtggcagcggacttgacagctactccccgctctttgtaacaacttgccagctttgcatttctcctctt
aaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaatgtgcaaagccggctttgctggagat
gatgctccccgtgctgtgttcccctctattgttggacgcccaagacaccagggtgtaatggttggtatgggccagaaggacagctatgtaggtgatgaag
cccaaagcaagaggggtatccttacccttaagtatccaattgaacacggaattgttacaaactgggatgatatggagaagatttggcatcacaccttcta
caatgagcttcgtgtagcccctgaagagcacccagtcctgcttacagaagctcccctgaaccccaaagccaacagagaaaagatgacacagatcatgttt
gagactttcaacaccccagctatgtatgtcgccattcaagctgtgctgtctctgtatgcctctggtcgtaccactggtatcgtgatggactctggagatg
gtgtcacccacacagtacccatttatgaaggttatgccttgccccatgctatcctgcgtctggacttggctggtcgtgacctgacagactacctcatgaa
aatcctgaccgaaagaagctacagcttcaccaccacagctgagaagagaaatgtgcgtgacatcaaggaagaaactctgctatgttgcctcttgacc
3' EST: CF784184
[+]tttttttttttttttatttactgcagcgctttattttatccttacagaacggtgtttntggaagttacaattctcatctggatgacatacaaattttggt
tttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaattaacttggatgcataaatttacaggtgttaactgcaacatttgtaactt
tcttcagacccattttgaatgttgggaatacaacctgggttaagaaaaagactggttcttgggcctgagtgtcaatttccaaccttactataaactggaa
agatagaagagattccttcatgaactgaccagcctccgacatgaaacaatggagtattaagcgcttaactgtgtgtggaagtctaaacctcagataccct
gtacacatctccaaaactcgacttctccataaacagcccctgtaacctagaggagaagtgtcattcgcaatactgtcgcagatcggcagcctttcacaga
aatgcttctttctacgtaaagcttagcttaagtgtcacccagactgtatgccaaccaggcaatataagagatgtgtacagggtacaatatgctagcattg
atgccacaggaatgatttgaagagacaagcacaagtcttgcattacaaaatcccccaacagtgactatgtgtgtctccaattcctaggataagttcattt
ggaagttggccctttttccagtttcaagaggctagcatgangcgtacacatttgccaaagcaaattaatacttttgaagaactcacgcagcaaatgcaac
gcatctgctagcagt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: