XB-CLONE-4352536
Clone Name: IMAGE:7027434
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Gene:
snw1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784186
[+]
taattaatcccccccccccccggtatttgtctccgaggtcacttcctcagtatcccagctgcgtttccggaaggaggacgaaagggaaaatggcgctggc
cagtttttgcctgctccaacgcagttatcacaggaccaattagaggcagaagaaaagctgagagcacagaagtctcgtcagaccgcgttagttgcatcgc
gccgtgaaccacctccctatggccacagaaaaggatgggtacccagatcccttgaggattttggagatggcggtgcttttccagagatacatgtggcaca
atatccactggacatgggccgcaaaaagaaaatgtccaatgccttggctgtgcaggtcgatgcagaaggcaagataaaatatgatgctattgctcgtcag
ggacagtccaaggataaggtcattttcagcaagtacacagacctggtacctaaagaggtgatggatgaagatgatcctgagctacaaagacctgatgagg
aaacaattaaagagctcacagagaagacaagacaggctctggaaaaatcagtgtcccaaaagattgctgctgcaatgcctgtacgagctacagataaatt
ggcacctgcacagtatattaagtacacaccctctcagcaaggagtggcttttaactctggtgccaagcagagggtgattcggatggtggagatgcaaaag
gatccaatggagccacctcgatttaaaattaataaaaaaattcacgtggggcctccatctccctcaagcaccgtaaagg
3' EST: CF784187
[+]tttttttttttttttccttctgcgccatcttacgctccacctgggctctcatttccactgcttctcgggcctttctgtcagctatgtacaaggcttcagc
aagcttagcaaagttctcattgatatggacagtctgcagcccccggccatcagcagccaaacgtttatctagtgggatggtataacccttggcattcttc
cagttagaaatacatgggggtattttccactcttgctgttcctttacagtcatctttctgctgggagaatgcattactggtgctggaggagatggaggcc
cacgtggaattttcttattaattttaaatcgaggtggctccattggatccttttgcatctccaccatccgaatcaccctctgcttggcaccagagttaaa
agccactccctgctgagagggtgtgtacctaatatactgtgcaggtgccaatttatctgtagctcgtacaggcattgcagcagcaatcttttgggacact
gatttttccagagcctgtcttgtcttctctgtgagctctttaattgtttcctcatcaggtctttgtagctcaggatcatcttcatccatcacctctttag
gtaccaggtctgtgtacttgctgaaaatgaccttatccttggactgtccctgacgagcaatagcatcatattttatcttgccttctgcatcgacctgcac
agccaagggcttgggacattttctttttgcggcccatgtccagggggatattgtgccacatgtatctctgggaaaacaccgccatctcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: