XB-CLONE-4352531
Clone Name: IMAGE:7027432
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Gene:
fh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784189 [+]
taattaatcccccccccccccgcccttcgacacgctgcgcggcacaggaaaaacctttctgcgctcgctgtacagtcattaacagtgaaacaggggagga
tggccagctcatacagaattgaaagtgatacatttggagaactccaagttccaaatgataaatattatggggcacaaactgttcgttcaacaatgaattt
caagattggaggtgtaactgaaagaatgccagttcctgttattagagcttttggcatcttaaaaagagctgctgctgaagtaaaccaagactatggactt
gatcccaacatcgccaattttattattaaagcagctgatgaggtttcatctggcaaattggatgatcactttcctttggttgtctggcaaactggatctg
gaacacagacaaacatgaatgtaaatgaagtaataagtaatagagccattgagatgatgggaggaaaacttggaagcaaagatcctgtgcaccctaatga
tcatgttaacaaaagtcaaagttcaaatgatacatttcctacagccatgcatattgctgctgtaaaagaggttcatgaagttcttttacctggactccaa
aagttacacgatgcattggaagaaaaatccaaggagtttgagaacattataaaaatttggtcgcacacacacaccaagatgcagtgccacttactcttgg
gcaggaaattgggtggttatgtttcaccaaataaaattccgtggttagtaggattaaagctgcatttacaacgggtc
3' EST: CF784190 [+]tttttttttttttttaccagatttgatgtatttttattaagacagcaatacaaattcaaatttattttggtccaagcatatcctctggtttaacccactg
gtcaaattgatctgatgtaagaaatccaagtttaattgcagtttcttttagtgtagtgccttccttgtgggcagttttagctatttttgctgctttatca
tatcctatatggggattaagtgcagtgacaagcattaaagactcgctcattaatttattgattctctctgtattggcttgtactcccacaatgcagttat
ctgtgaatgacacagctgcatcgcccagcagcctgattgattgaaggacattctttataattacaggcttgaaaacatttaattcgaagtggccattgct
gcaacctattgttatagccacatgattccccatcacttgggctgcaaccatagtaacagcctcacactgtgtaggatttacttttccgggcataatgctg
ctccccggttcattttcaggcaggattagttctccgagacctgatcttggtccggacccaagaaaccgtatgtcattagcaatcttcataagactacatg
ctagtgtattcaaagctccacttaattcaacgagtgcatcatgagcagcaagggattcaaatttattaggtgcagtaataaatggcagaccccgttagct
ctgccacttttgcagcaactttttctgcaaatccaattctgggtgtttaagcccgtttccaactgctgtaactccagctgctaattcataa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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