XB-CLONE-4352528
Clone Name: IMAGE:7027431
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Gene:
qrich1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080472 [+]
TGAATGTAAGATGGCGCCCAGGGAGCTGTGAGGCGAAAATAATCTATCGGAAAAAAAGGAATAATAATCACCCGAAGAGGGAGCGGGAGGACTGAGCAGA
GCCCGGGGAGCCAAGTGTGTGGCAGCGGCACCGGAGGAGGAGAGGGCCAAGCGGTGCCGGAGTGAAGCTCCCTCCTGACAAGGAGCGGGCGGAGCAGCTG
GGGATTTGACTCAGTTGTGTACGCTATGAATAATTCTTTGGAGAACACCATTTCGTTTGAAGAATATGTCCGCATGAAGGCCCGTACAGTTCCACAGCAC
AGGATGAAGGAGTTCCTGGAGTCACTGGCCTCAAAGGGACCTGAAGCCTTACAGGAATTTCAGCAGCCACCACCCACCACCACCATGGTCTATCAACAAG
GGGCTAATTGCATCTATACAGACAGCACAGAGGTGGCAGGATCCTTACTTGAACTGGCTTGTCCTGTTACAAGTGTGCAGCAACAGCAAGTGCAGCAGGA
ACAGCAGATACAAGTTCAAGCACAACAGGTGCAGATTCATGAAGCACTCACCAGGCCTTGGAATCTTCTCCACCCTGCCCCACAGCTGAATCGAATGAAC
CTCTCCCCCGAGGGGTCCCAGGATTCTGGTATTTCAGAAGACATGTTGGTGCAGGTACAGCAGTCACCGCAGCAGGTAACGGGTCAACAGCTCTCGCCAC
AACTTAATGTTCATCCATCAAATGAGCAGCCAATTCAAGTGCAAGTACAAATCCAGGGTTCAGGACAGTCACAAACAGCGCAAACAATACAGAATCAGTC
CCTGCAGAGCCAGACCCCTTCCCAGATGCAAGTGGCCCAGATTCAGGTCCAGCATGTTCAGGCTGGCCAGCAAATTCAGCCCTCTGATATGCAGGACGAT
CATATACAGCATCAGCAGATCCAAGCACAGCTAGTGGCAGGACAGTCTGTGGGCAGCGGTATCCAGATACAGCAAACAGCCAGTGCTCTTTCCCCTCCAC
CCACTCAGCAAGGCTCCCCACGGGAAGTGGAGCGCCGATCCAGCTCTTCGAGTGTCCTTCAGCCAGTAAAGAAGCGCAAAGTGGATATGCCAATCACTTT
GTCATATGCAATCTCAGGACAGCCTGTTGCAGCTGTTTTGGCAATTCCACAAGGGCAGCAACAGAGTTATGTGTCTCTGAGGCCAGACTTACTAACAGTA
GACAGTGCCCATCTCTATGGGGCCACTGGGACCATTACAAGCCCAACAGGAGAGACGTGGACCATCCCGGTCTACTCTGCTAATGCCCGCAGTGACCTAC
CGCAGCAGAACATAACCCACATAGCCATCCCTCCAGAGGCCTACAATGCTGTCCACGTTACAGGTTCCCCCACTGTGGCGGCTGTTAAATTAGAAGAGGA
GAAGGACAAAATGGTCAAAAATACACACGAGGAAGTGGTGCAGACTCTTGCAAATTCTCTGTTTCCAGCTCAGTTCATGAATGGGAATATCCACATTCCA
GTGGCTGTCCAGGCTGTGGCAGGGGCATACCAGAATACAGCACAGACTGTACATATTTGGGACCCTCAACAGCAAGCAACGCTACATGAGCAAGTTCAGC
AGCATCAACAACAGCAACAACATCAGCTGCAGGCACAAGCTGTTAACGAAGTAGAACCACAACAGCAACCTTCTCCTGAACTTCTGCTTCCCAGTACCTT
GAAACCAGAGGAAGGGCTTCAAGTATGGAAGAACTGGGCACAGACAAAGAATGCAGAGCTTGAAAAGGACTCCCAAAACAGATTAGCACCCATAGGAAGA
CGTCAGCTCCTAAGGTTTCAGGAAGATCTCATTTCCTCGGCAGTTGCAGAGTTAAATTATGGGCTGTGTCTAATGACTAAAGAGGCTCGCAACATGGAAG
GTGAACCCTATGATGCTGACGTTCTCTACTACATTTTTCTGTGTATACAGAAGTACCTGTGTGAGAATGGAAGAGTAGACGACATATTTTCTGACAACTA
CTACATCAAGTTTACAGAGTGGCTTCATGAGGTTCTGAAAAATTATCAGCCCAGAGTCACTCCTATTGGATATGTGATCCCCAGCCATGTGACCGAGGAA
ATGCTTTGGGAGTGTAAGCAGCTGGGCGCCCACTCTCCTGCCACACTACTTACAACACTTATGTTTTTTAACACAAAATATTTTCTGTTAAAAACTGTTG
ACCAGCACATTAAATTGGCCTTCTCCAAAGTCCTAAGGCAAACGAAGAAAAATCCTTCCAATCCAAAGGATAAGAGCACGAGCATCCGGTACCTGAAAGC
ACTTGGGATCCATCAAGCAGGGCAGCAAAAAGGTTTCTCAATAACAGACGACATGTATGCAGAGCAAACAGAGAACCCAGAAAATCCATTACGATGTCCC
ATTAAATTGTATGACTTCTACCTCTTCAAATGCCCACAGAGTGTAAAAGGACGAAATGACACATTCTATTTGACGCCAGAACCAGTCGTAGCACCCAACA
GTCCAATCTGGTATTCTACACAGCCAATCAGCAGAGAGCAAATGGAACAGATGCTAACCCGAATTCTGCTCATCCGAGAGATCCAGGAAGCCATTGTGGT
TGCGAGTGCCAGCAACATGCACTAAATCATTCTTTCTGGGCACAGAAGTCATGTAAAAGGAAGAGAGGAAATAAAGAAACCTGTACAGACTTTTACACTA
TGAGAAGATGCCTACTTTTTCTGTTATTTTTATTTTATTTTTTATTGGTGTAGTTATGGAGCCCTTTTAGGCTTCTCCTGTTTAAAATAAAGTGTAAGTA
AAAGCGCCCAAATGTGCATTTAAAGACAAAAAAAAATCTGCGTTCCTAAATGGCTGGAAAGCGAAACGGTTATTGTTATTTTAAACCTTTTTCCCCCCCA
CCCCATATCCTTTTAGCATGGTCTAGAAATATTTTATTTTATTTGGGAAAAGAAAGAAGGCATCTTAGGTTGAATGGGATGGACTGTGCAGCTGCTCACG
CCATTGCCTACCACATATATGGACGTTTATTCTGTATTTTCAGTTGCAGGGCCAAGCGACCCCTGGTTTTATATAACTACATATATAAAAATATTTCAGA
AAAAGTCGGATTATTTCACATTAACCTGAATATATTTCTTTTTTTTTTTTTTTTTCCTGGCCCAAATTATCAAGGCCTTAAACTGCAGGCATATGAGCCT
ATGTAAATTTATTGTAATATAATCTTGTGTATCTGCTGCAGATATCTCCATATTTCTCCATTGTTCTTATGCTCTTTGTTTTATTAAGAATGACAAGCAC
GTCTTTTTAACATGAAAAAAAAAAAAAAAA
5' EST: CF784192 [+]taattaatccccccccccccctgaatgtaagatggcgcccagggagctgtgaggcgaaaataatctatcggaaaaaaaggaataataatcacccgaagag
ggagcgggaggactgagcagagcccggggagccaagtgtgtggcagcggcaccggaggaggagagggccaagcggtgccggagtgaagctccctcctgac
aaggagcgggcggagcagctggggatttgactcagttgtgtacgctatgaataattctttggagaacaccatttcgtttgaagaatatgtccgcatgaag
gcccgtacagttccacagcacaggatgaaggagttcctggagtcactggcctcaaagggacctgaagccttacaggaatttcagcagccaccacccacca
ccaccatggtctatcaacaaggggctaattgcatctatacagacagcacagaggtggcaggatccttacttgaactggcttgtcctgttacaagtgtgca
gcaacagcaagtgcagcaggaacagcagatacaagttcaagcacaacaggtgcagattcatgaagcactcaccaggccttggaatcttctccaccctgcc
ccacagctgaatcgaatgaacctctcccccgaggggtcccaggattctggtatttcaaaaaacatggttggtgcaggtacagcattcaccgcaacagggt
aacggggtcaacagcttctcgccacaatttaatggttcatcccttcaaaatgaagcagcccatttccaggtggcaagttaccaatc
3' EST: CF784191 [+]tttttttttttttttcatgttaaaaagacgtgcttgtcattcttaataaaacaaagagcataagaacaatggagaaatatggagatatctgcagcagata
cacaagattatattacaataaatttacataggctcatatgcctgcagtttaaggccttgataatttgggccaggaaaaaaaaaaaaaaaaagaaatatgt
tcaggttaatgtgaaataatccgactttttctgaaatatttttatatatgtagttatataaaaccaggggtcgcttggccctgcaactgaaaatacagaa
taaacgtccatatatgtggtaggcaatggcgtgagcagctgcacagtccatcccattcaacctaagatgccttctttcttttcccaaataaaataaaata
tttcaagaccatgctaaaaggatatggggtgggggggaaaaaggtttaaaataacaataaccgtttcgctttccaaccatttaggaacgcagattttttt
ttgtctttaaatgcacatttggggggcttttacttacactttatttgaaacaggagaagccaaaagggctccataacttcgccatttaaaaagaaaatta
aactatctgaaaaagtaggcatcttctcaaaggggataagtttgttaaggtttcttttattccctcccttcccttttagatgacttccggggcccagaaa
agaagaatttagttggatggttgtctggccactcggcaaccccaaaggggctttccagggaatccccccggaagaaacaaaaaattcggggttaagcaac
ttggttcccagtttggctcttccggatgtaattgggcctgtggttaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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