XB-CLONE-4352522
Clone Name: IMAGE:7027429
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Gene:
stt3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784196
[+]
taattaatcccccccccccccgagcagagggagaggagcggctgccaggtttctagtgttttctgtcccttccctttatgtaaaatgactaaattaggat
ttttgcgtctgtcctatgagaagcaggacactctcctcaagctcctcatcctgtctatggcagcaattctctccttctctaccagattgttctcggtttt
gagatttgaaagtgtcattcatgagtttgatccgtatttcaattatcgaacaacaaggtttcttgccgaggaaggattttataatttccataactggttt
gatgatcgagcatggtatccattgggaagaattataggaggaacaatctatccaggtctgatgataacctctgcagcattctaccatattctacactttt
tccacatcactattgacatccgaaatgtttgtgtattccttgctccactcttctcgtctttcaccaccatagtcacttaccatctgacaaaagagctgaa
agatgctggtgcaggccttttggcagcagcaatgatagcagtggtacctggctatatatcacgttctgtggctggatcctatgataatgaaggtattgcc
attttctgtatgcttctaacttactacatgtggaataaagcagtaaagactggttcaatctattgggcagccatgtgcgcttctacatatttccataggg
tctcctcggggggaggatacttgttttcgatcaaccttaaccacttcatgtccctggttttgatgcttaccggaagattctctcaccaaatttagtgggt
ttatggaaa
3' EST: CF784195
[+]tttttttttttttttgtttccccatctttaattgaccacggtaaagtcagaacaacaaaaaaatcatttntggtagcattcatattcataaaaagcattc
cacccaacatcgacagcaaccagtgtgacttccacagccaaataagttctttctttccgtcaatagaaacagtataaaaaaaatcttctggttcacaggt
gaaggctctttcagtgctgtttgtattaaagctgaaggcaaaaaaaattatgttcttgataaccctcgattatccagatcttttactttatatattctga
caagccagtgctctgtggtgtaagcttcctctagaacatcaagctcgaaatctttattgccgatttctgcgtttctcactctgtcataacctggagggcg
tttggcttcggtgtagacttgaccaaagcgataatagcacatcttgtacataaggcagttgagcagaacgggagatccttctctatctacacgaaactct
ccagtaggagtgtagtaatcgtgctctttgatgtgtttaccagtgtcagtgcttcctccaattcttaccatccacaaaaatttgttgatatcatctgatg
aatatcctgtgaggccccccaaaataacaaggacataactaacatccagctctctcataatctcataggctttttcttcagtggatgccattgccctggc
ctactctgggaaaagtgtgttgttattccagggatttattatccaaccaaggattgttccgaatggcccattgccttgtaacctgggtaacccataaatc
ccccccaccgacatttaactttttgcatcccctcaaggggttggttgttgtca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: