XB-CLONE-4352514
Clone Name: IMAGE:7027426
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Gene:
uevld
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080471
[+]
CCCCCCCGCTCTTCCCAACCCCTCCCTGCCCTATTTCCCATGAGCCAGTGCGGCCGCTTCCCATCATGCTCCTGGGAGGGCCGCCATGGAGTTCAGCGCA
GAGACGCTCCGCCAGCAGCTCGGCAAGTATAAATTCCGAGATCTCACCATCGAGGAACTGAAGGACCTGAACAGAACCTTCCCCTCCTTCATCTTCTCAA
TGGAGACCTATACATTTAGAGACGGATCCCAGAAGGACCTGCTCAACCTCACAGGGACTGTTCCCATGAAACACCAGGGCACAACGTATAACATTCCCAT
CTGCCTGTGGATTCTGGACTCCCACCCATTTGCCCCCCCACTCTGCTTTTTGAAACCGTCCGGTAACATGGGGATCCGGGTTGGGCGACACATCGATGCG
CAGGGAAGGATTTATCTGCCGTATTTACAGAACTGGAGCCATCCCAAGTCCACGGTCACTGGCCTCATACGGGAAATGGCGGTGAAGTTTGAGGAGGAGC
TGCCATTGTACTCACTGTCGGCTGAGGATGGCGCCAGGCAGAGGGAGTTACTGTCCTACATTGCCCAGGTTACGGATGGGGTTTCCAGTATGGATGTAAA
GGCGCCAAGTCGTGCCAAGGTCACTGTGATTGGCGGAGGAGATATGGCACTGGCCTGCTTATTGGCTGTATCTGCGAAGGGCACCGCAGGGAAACTGCTT
CTTCTCGACCCGACAGATGGTGAAGCCGCAGGAGGAGCAGCTGCCGACCTTGAAATCTTCAGCCTCCCAAATGTACAAGTCACCAAAGATTTCTCTGCGA
TCGCCGGCTCTGCCATAGTGATTGTCACGGTCAATTCCTGGAGCAACTCCCAGTCCTACGTTGGCGTCCTACAGAGCAACGTGGAGCTGCTGCGTGGCAT
TCTACCCGCCGTGGCACATCACTGCCCCAAGTGCCTTCTACTGGTGGCCTCACAGCCAGTTGAGATCATGACTTACGCCACATGGAAGCTGAGTGGGTTC
CCTCACAATCGAGTTCTTGGCATCGGCTGTAACTTGGATTCGGGGCGGTTCCGCCATGTGATTGAGAAGTTGGCGGATTCTGAGGAGGGGGCACAGGGAG
CCTGGATTATTGGGGAGCAGAGTGACAACAAAGTGGCTGTTTGGGGGGCTCCAGACTCATCTGCCAACCGTCAGACTCCCTGCAAATTGTACCCCAAAAT
CTTCCAGGAGCAGCTGACAAGCAGAGCTTTGGAGATCCTTAAAGGGAAGGGGCAGCGCTCATGGTCTGTCGGCCTCTCTGTGGCTGATATCACCGACACC
CTCGTACAGAATAAAGGCAAAGTACATTCTGTCTCTGCACTTTGTAAGGGACAATTCGGGGTGCAGGAGGAGGTGTTTCTCAGCATCCCGTGCGTATTGG
GGAGCGCGGGGGTGACGGGGGCGGTACAGACGCTGCAGGACGAGGCTCAGATTTGGGAAACGTTACAGAGAAGTGCGGCAGCCATAGAATCTGTGCAGCA
ACAGCTGAGACTGTGAGGGGGCGGGACCCTGCGATGGCCAATCCCGTTGAATGCTGGGAACTCATTTTGGGAAGGTTCCAGACTGGAGGTGGAGCTTCTC
CCTTTATTCGCCAATCAGATTTGTTGTCTTTATTTTTATACCAAGAATGTACATTTTTTTCTACAGACTCTTCTGTGTTGGGGGAATCGTGGTGCTGCTT
TATACAGCCAATCATACAGCTGCTCTCTCTCCTTGACTGGGCACATGGTTTGTCTGCCCATTGTTTAATAAATAGCTGCTGGTGCATTCTGGGATAAGGG
CACTGGTTATTATGGGATGTTCCCCCCTAACCATAGTGGCAACTATCTGGCTATTGGGGGTTGCTGGGAGTTTTAGTTTAACATTATCTGAGGGATCTCC
ATTTGCTTATTTCTGGCCTATAAATAAAGGGCAAGTTCACCCCTGTGTGGGAGGTACTGGCACAGGCAGCGGGGTAACTATACAAACCCTGGGAATGGCG
CCCTCTGTGGGGTATAAGGGCCACTGTGCCCTGGCATTCTGGGAGCTGCTATACACTCACAGTAACATAGTGTGTATATATATATATATATATATATATA
TATATCGTGATCCTACCCCCATTAACTGCCCCTTCCCTAGTGACGAGGTTCCCCCAACCCATAGGTACCATAGCAGCTGAGAGTGAGTGTGAGTGAGTGT
GAGCGAGTCTGAGTGAGTGTGGCTAGCACTGCACTCAGCTCTCTCAGTAGCCGGGGGTGCATCCCATCTGGCCCTGGGGCCTTGTTCACAAGGGGTAGTT
CACAAGGGGTAGTTCACAAGGGGTAGTTCACAAGGGGTAGTATGTTACAGAATGCCCCATTTCCAGCACCATTGCAATTGGTTTCATTGATTTATTTTTT
ATAGTTTTTGAAATATTTCTCCGCCTCCTTTCAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF784200
[+]taattaatcccccccccccaacccccccgctcttcccaacccctccctgccctatttcccatgagccagtgcggccgcttcccatcatgctcctgggagg
gccgccatggagttcagcgcagagacgctccgccagcagctcggcaagtataaattccgagatctcaccatcgaggaactgaaggacctgaacagaacct
tcccctccttcatcttctcaatggagacctatacatttagagacggatcccagaaggacctgctcaacctcacagggactgttcccatgaaacaccaggg
cacaacgtataacattcccatctgcctgtggattctggactcccacccatttgcccccccactctgctttttgaaaccgtccggtaacatggggatccgg
gttgggcgacacatcgatgcgcagggaaggatttatctgccgtatttacagaactggagccatcccaagtccacggtcactggcctcatacgggaaatgg
cggtgaagtttgaggaggagctgccattgtactcactgtcggctgaggatggcgccaggcaaagggagttactgtcctacattgcccaggttacggatgg
ggtttccagtatggaagtaaaggcgcccagtccgtgccaaggtcactgtgaattggcggaagaaaaatgggaactggccctggcttaatgggcggatctt
gcgaaaggggacccgcaggggaaaaatggtttcttcttcgaacccgaaaagaagggtgaaaaccccccggggaggaaacaacctgcc
3' EST: CF784201
[+]tttttttttttttttcttttttttgaaaggaggcggagaaatatttcaaaaactataaaaaataaatcaatgaaaccaattgcaatggtgctggaaatgg
ggcattctgtaacatactaccccttgtgaactaccccttgtgaactaccccttgtgaactaccccttgtgaacaaggccccagggccagatgggatgcac
ccccggctactgagagagctgagtgcagtgctagccacactcactcagactcgctcacactcactcacactcactctcagctgctatggtacctatgggt
tgggggaacctcgtcactagggaaggggcagttaatgggggtaggatcacgatatatatatatatatatatatatatatatacacactatgttactgtga
gtgtatagcagctcccagaatgccagggcacagtggcccttataccccacagagggcgccattcccagggtttgtatagttaccccgctgcctgtgccag
tacctcccacacaggggtgaacttgccctttatttataggccagaaataagcaaatggagatccctcagataatgttaaactaaaactcccagcaacccc
caatagccagatagttgccactatggttaggggggaacatcccataataaccagtgcccttatcccagaatgcaccagcagctatttattaaacaatggg
cagacaaaccatgtgcccagtcaagggagagagagcagctgtatgattgggctgtataaagcagccaccacgattc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: