XB-CLONE-4352505
Clone Name: IMAGE:7027422
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Gene:
slc34a2
,
ppan
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080470 [+]
AGTGGAAGCTGAAGCGCTGTTACACGTGTGTGCCGGAGCAGCACTTCTGCCAACATGGGGAAAGATAAGACCAAGAATCAGAAGAAGCTGAAGGTTGCAG
CCAATCACAACGCAGAGGAAGAATTTAATGCTGTGCCGCACTCTTTCGTTTTCCAACGAGGGCAGATTGGAAAGAATGTGCAGCAGCTCATCGTGGATGT
CCGGAGAGCCATGGAGCCCTTTACTGCCCGCTCTCTAAAGGTTCGGAAGAAGAACACACTGAAAGACTTTGTGGCAGTGGCGGGGCCCCTAGGAGTTACT
CATTTCATGATCTTCACCAAAACCACAACCAATGTCAATTTCAAATTTGTTCGCTTGCCCAAAGGACCTACATTAAGCTTCAAAGTGAACCAGTACTCTC
TTATGAAGGACGTGGTCTCCTCTCTGAAGAAGCACAGAATGCACGAGGAGCAGTTTATGCACCCACCCCTACTGGTGCTTAATAACTTTGGCAACCAAGG
AATGCACGTCAAGTTGATGGCCACCATGTTCCAGAATATGTTCCCTTCCATTAATGTGCACAAGGTAAACTTAAACACCATCAGGAGGTGCATTCTCATT
AACTACGATGATGAAACACAGCAACTGGACTTCAGGCATTACAGCCTGAAGGTGGTACCAGTTGGCATGAGTAAAGGAATGAAGAAACTCCTCCAGGAGA
AATTTCCCAACATGAGCCGCTGGGAGGACATCAGTGAGCTCCTGGTAAAAGGAGCCAACCTGTCTGAAAGTGAAGCTGAGCAGGACGGGGACCACAACAT
AACGGAGCTGCCGCAGGCGTGCGCGGGACGCGGGAACATGAAGGCTCAGCAGAGCGCCGTAAGACTGACTGAGATTGGGCCCCGGATGACTTTACAGCTG
GTGAAAATAGAAGAGGGTCTAAGTGACGGCAAAGTGTTATATCACGGCTTCATTCAGAAGACAGAAGAGGAGATCAAGGCCATGTTGGATAGCAGAGAGA
AGAAACTCCAGCGGAAGAAAGAGAGGAAGAAGAAGCAAGAGGGAGATGTTGAACGCAAGAAGAACCTGAAGGAGGAGAACAAGAAGCGCAGCTTAGCCGG
AATGAAGAGGAAGCGCGGGGACGGGGAGGCCAGTGACAGTGAAGTGGAGGATCCTGGTACTTTGGGAGATCAGGATCCAGCTGACCAATCAGAAGATGAT
GATGCTGAGTATTACAGACAGGAAGTTGGAGAGGAGCCAGATAAAGATCTCTTCCTCCACACCAAGAAAAGGTCCAGCCAGAAACCTGGGCCTCCCAGAA
AGAAATTCAAAGGGTCTGGAAAAGAGAAGCCAAAGTCCGACACGCGTGGGGGGCACAACAGGCCTCACAGCGGCAAATCACCCATCCAGAAACGTGGAGC
CAATGTGGGAGGGAAAGCCTTTAAATCTGCTCACAAACTGAAAGGCCAGAAATTCCAAGGAAAGGGAACTGAGCACAGACCCCAGCAAGGAGGCAAAAAG
TTCCCCGGCAGTAAAAGGGGGTTCGGACCTAAGAGCCCCAAGACTGGGCATAAACCCAAAGGGCCGGGGCATAAACCCAAAGGGCCGGGGCATAAACCCA
AAGGGCCGGGGCATAAACCCAAAGGGCCGGGGCATAAACCCAAAGGGCCGGGGCATAAACCGGCAGACAAAAGAGGCAAACCCTTCAAGCAAAAGAGAAA
GTGACTTGCTGGGAATGATCTGATGGACTCCTTGTAAATAAGAGTGTAATTATGTGCCCCTCTCCTTGTATTTTGTCACTGAGCTCTGAAATAAAACTGC
CCCGCTGTTACTAAAAAAAAAAAAAAAA
5' EST: CF784206 [+]taattaatcccccccccccccagtggaagctgaagcgctgttacacgtgtgtgccggagcagcacttctgccaacatggggaaagataagaccaagaatc
agaagaagctgaaggttgcagccaatcacaacgcagaggaagaatttaatgctgtgccgcactctttcgttttccaacgagggcagattggaaagaatgt
gcagcagctcatcgtggatgtccggagagccatggagccctttactgcccgctctctaaaggttcggaagaagaacacactgaaagactttgtggcagtg
gcggggcccctaggagttactcatttcatgatcttcaccaaaaccacaaccaatgtcaatttcaaatttgttcgcttgcccaaaggacctacattaagct
tcaaagtgaaccagtactctcttatgaaggacgtggtctcctctctgaaaaaacacagaatgcacgaggagcagtttatgcacccacccctactggtgct
taataactttggccaaccaaaggaatgcacgtcaggttgatggccaccatgtttccaaaaaatgttcccctccatttaagtgcacaaggtgaaatttaaa
cccctcagggagggcatttctcataaacctacgaatgaagaaaaccagcaactgggacttcaggcattacaagcctgaaaggggggaccccatttggcct
tggggtcaagggagatgaagaaacctcccctccaggaaaaaattttctccccaaaatgaatcccgcttggggaggtaaaattaaggtaaggccccctggt
taaaaaaggaaccn
3' EST: CF784207 [+]tttttttttttttttagtaacagcggggcagttttatttcagagctcagtgacaaaatacaaggagaggggcacataattacactcttatttacaaggag
tccatcagatcattcccagcaagtcactttctcttttgcttgaagggtttgcctcttttgtctgccggtttatgccccggccctttgggtttatgccccg
gccctttgggtttatgccccggccctttgggtttatgccccggccctttgggtttatgccccggccctttgggtttatgcccagtcttggggctcttagg
tccgaacccccttttactgccggggaactttttgcctccttgctggggtctgtgctcagttccctttccttggaatttctggcctttcagtttgtgagca
gatttaaaggctttccctcccacattggctccacgtttctggatgggtgatttgccgctgtgaggcctgttgtgccccccacgcgtgtcggactttggct
tctcttttccagaccctttgaatttctttctgggaggcccaggtttctggctgggacttttcttggtgtggaggaagagatctttatctggctcctctcc
aacttcctgtctgtaatactcagcatcatcatcttctgattgggcagctggaatcctgatctcccaaagtaccaggatcctccccttcccgggcctgggg
ctccccgtccccgcgcttcctctttaattcgggctaaactgcgcttccttggtccccccctttcaggttccttcttgcgtttaaaaaaccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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